【作者】 朱晓茹； 赵守亮； 唐荣银； 张蓉； 李玉成；

【Author】 ZHU Xiao-ru, ZHAO Shou-liang, TANG Rong-yin,ZHANG Rong. LI Yu-cheng. (Department of Operative Dentistry and Endodontics, College of Stomatology,The Fourth Military Medical University.Xi’ an 710032; Shanxi Province ,China)

【机构】 第四军医大学口腔医院；

【摘要】 目的:建立人牙切片体外器官培养模型。方法:收集年轻人健康前磨牙或第三磨牙,用慢速切锯制备2mm厚的牙横切片,将切片用含1％琼脂糖的半固体培养基包被后采用Trowel器官培养方法在体外培养2-14天,对培养切片的细胞及组织形态进行组织学观察。结果:实验成功的将牙切片在体外培养了2周,成牙本质细胞在培养一周之内能够很好的维持原来的形态。 1周之后成牙本质细胞逐渐萎缩,细胞密度减小。结论:实验成功的建立了人牙切片体外器官培养的模型,为后期进行组织损伤和修复、第三期牙本质的形成等方面的研究奠定了基础。更多还原

【Abstract】 PURPOSE: To establish human tooth slice organ culture model in vitro. METHODS: Young healthy human premolars and the third molars were collected, and cut into 2mm-thiek transverse slices by low speed diamond-edged saw. The slices were embedded in 1% semisolid agarose-based medium and cultured in Trowel-type cultures for 2 to 14 days. Preservation of cell and tissue morphology was observed. RESULTS: Tooth slices were successfully cultured in vitro for two weeks, and the odontoblasts could maintain their original morphology within one week. After one week, the odontoblasts gradually shrank, and the cell density decreased. CONCLUSION: Human tooth slice organ culture model was successfully established, which provided a better method for further study on dental tissue repair after injury.更多还原