【作者】 王效英； 张旗； 陈智；

【Author】 WANG Xiao-ying, ZHANG Qi, CHEN Zhi. (School of Stomatology.Wuhan University. Wuhan 430079.Hubei Province.China)

【机构】 武汉大学口腔医学院牙体牙髓科,口腔生物医学工程教育部重点实验室；

【摘要】 目的:研究LIM矿化蛋白1在正常及炎性人牙中的表达情况。方法:利用免疫组织化学技术检测并比较LIM矿化蛋白1 在正常及炎性人牙中的表达,SPSS11．0软件分析结果。结果:在正常人牙中,LIM矿化蛋白1主要分布于前期牙本质、成牙本质细胞及血管内皮细胞。在牙髓炎人牙中,LIM矿化蛋白1主要分布于前期牙本质、成牙本质细胞、未矿化的修复性牙本质、成牙本质细胞样细胞、牙髓成纤维细胞及血管内皮细胞。LIM矿化蛋白1在炎性牙髓成纤维细胞中的表达要显著性高于正常牙牙髓成纤维细胞。结论:LIM矿化蛋白1可能在人牙本质矿化过程中发挥一定的作用。更多还原

【Abstract】 PURPOSE: To investigate and compare the expression of LIM mineralization protein-1 in healthy and inflammatory human teeth. METHODS: Immunohistochemistry was used to localize the expression of LIM mineralization protein-1. RESULTS: In healthy teeth, LIM mineralization protein-1 staining was observed mainly in the predentin, odontoblasts and endothelial cells. In teeth with pulpitis. immunoreactivity was mainly observed in the predentin, odontoblasts. unmineralized reparative dentin, odontoblast-like cells, human dental pulp cells and endothelial cells of the blood vessels. Statistical analysis showed that the expression of LIM mineralization protein-1 in fibroblasts in teeth with pulpitis was upregulated significantly compared with that of healthy teeth (P<0.05). CONCLUSION: LIM mineralization protein - 1 may play a crucial role in the mineralization of dentin formation.更多还原