【作者】 刘辉； 王强； 朱峰； 罗平平； 刘天麟； 韦晓玲； 汪丽丽；

【Author】 LIU Hui,WANG Qiang, ZHU Feng ,LUO Ping-ping, LIU Tian-lin ,WEI Xiao-ling. WANG Li-li.(Department of Operative Dentistry and Endodontics,School of Stomatology,Tongji University.Shanghai 200072;Department of Dentistry, Affiliated Kunshan Hospital, Jiangsu University. Kunshan 215300 ;Department of Dentistry, Affiliated Eastern Hospital, Tongji University.Shanghai 200120,China)

【机构】 同济大学口腔医学院口腔内科； 江苏大学附属昆山医院口腔科； 同济大学附属东方医院口腔科；

【摘要】 目的:研究过量氟对大鼠切牙碱性成纤维细胞生长因子(basic fibroblast growth factor hFGF)表达的影响,从分子水平探讨氟斑牙的发病机制。方法:20只Wistar大鼠随机分为2组:对照组(饮用蒸馏水)和实验组(饮用100mg／L氟化水),复制氟斑牙动物模型,8周末处死动物,获取切牙标本,免疫组化染色观察bFGF在大鼠切牙的表达定位及在对照组与实验组切牙表达的变化。结果:bFGF在分泌期成釉细胞,成牙本质细胞均呈阳性表达。实验组hFGF的表达明显弱于对照组,差异有显著性(P< 0．01)。结论:过量氟可抑制bFGF的表达,从而影响上皮与间充质之间的相互介导作用,导致釉质发育障碍。更多还原

【Abstract】 OBJECTIVE: To investigate the effects of overdose fluoride on the expression of bFGF in rat incisor. METHODS: 20 Wistar rats were randomly divided into 2 groups : Group Ⅰ (control group) distilled water was given :Group Ⅱ (experimental group) 100mg/l NaF was given;the rats were killed at the end of 8th week ,lmmunohistochemical staining was used to studv the expression of bFGF in rat incisors. RESULTS: Immunohistochemieal results demonstrated the presence of bFGF in ameloblasts. odontoblasts of rat incisor ,the expression of bFGF was reduced in group Ⅱ (P<0.01). CONCLUSION: The overdose fluoride inhibits the secretion of bFGF. effects the interactive between dental epithelium and dental mesenchyme,which leads to the enamel demineralization.更多还原