【作者】 张磊涛； 黄洪章； 曾东林； 张彬；

【Author】 Zhang Lei-tao, Huang Hong-zhang, Zeng Dong-lin, Zhang Bin Department of Oral Maxillofacial Surgery ,the Second Affiliated Hospital, Sun Yat-Sen University

【机构】 中山大学附属第二医院口腔颌面外科；

【摘要】 <正>目的:建立人成釉细胞瘤的裸鼠皮下移植瘤模型。方法:采用组织块直接贴壁法将新鲜成釉细胞瘤组织进行原代细胞培养,反复贴壁法和胰酶消化法使之纯化。然后将纯化的成釉细胞瘤细胞接种于裸鼠颈背部皮下。分别观察接种成瘤后瘤组织大体生长情况以及常规病理切片观察瘤细胞的侵袭生长情况。更多还原

【Abstract】 Abstract: Objective: To establish an subcutaneous xenotransplanted tumor model of human ameloblastoma in nude mice. Methods: Ameloblastoma cells were absorbed by primary culture , repeat attachment and pancreas proteolytic enzyme were both used to purified them. Then, the purified cells were implanted into the subcuits of nude mice. The specimens were respectively investigated by microscope in different spots after implanting . Results: Ameloblastoma cells can survive in all of the 8 nude mice. The xenograft can be found on 23 days after implanting. The rate of successful inocutation is 2/8.The subcutaneous xenotransplanted tumor cells can be found with microscope in the inter-muscle tissues of nude mice. Conclusion: The subcutaneous xenotransplanted tumor model of human ameloblastoma in nude mice was successfully established and it may benefit to further studies on this tumor.更多还原