【作者】 白明海； 吴汉江；

【Author】 B AI Ming - ha, WU Han - Jiang Department of Stomatology, Xiangya Second Hospital, Central South University ,Changsha 410011

【机构】 长沙市口腔医院正畸科； 中南大学湘雅二医院口腔科；

【摘要】 <正>目的:探讨牵张力对体外培养的不同年龄兔鼻软骨细胞增殖活性的影响及牵张力大小与兔鼻软骨细胞增殖活性改变的量效关系。材料与方法:将第4代体外培养的新生及6周龄新西兰白兔兔鼻软骨细胞置于细胞膜式牵张力施加装置上培养,流式细胞仪检测不同的牵张力(5kPa、10kPa)在0-12h内对兔鼻软骨细胞增殖活性的影响。结果:0-10h内5kPa牵张力组软骨细胞增殖指数随着牵张力作用时间的延长不断上升,增殖指数峰值位于10h处;0-8h内10kPa牵张力组软骨细胞增殖指数随着牵张力作用时间的延长不断上升,增殖指数峰值位于8h处;5kh较10kPa牵张力对体外培养兔鼻软骨细胞具有更大的更多还原

【Abstract】 OBJECTIVE The purpose of this study was to investigate the effects of distraction on proliferation of rabbit nasal chondrocytes in vitro and the relation between distraction and proliferation of rabbit nasal chondrocytes. MATERIALS AND METHODS The fourth - passage chondrocytes were harvested from the nose of neonatal and 6 -week -old rabbits. A continuous static distraction was applied on the cells in vitro using a cellular static distraction device, and the proliferation of nasal chondrocytes were examined using a flow cytometry. The experimental group were divided into seven groups under continuous static distraction (5Kpa,10Kpa) for 0 to 12 hours. RESULTS The results showed that the proliferation of nasal chondrocytes was promoted as the application time of static distraction was prolonged (0-10 hours in 5Kpa groups and 0-8 hours in 10 Kpa groups). The maximal proliferation appeared, when the nasal chondrocytes were cultured under 5Kpa continuous static distraction for 10 hours , and 8 hours under 10 Kpa distraction (P < 0. 05). The proliferation of cells in the 5Kpa group was more obvious than in the 10 Kpa group (P <0. 05). The proliferation of cells in the neonatal rabbit group was more obvious than in the 6 - week - old rabbit group (P < 0. 05). CONCLUSIONS The date prove that static distraction stimulates in chondrocytes can influence and regulate the growth of nasal chondrocytes. It might provide a new methord for the the therapy of nasal deformity of cleft lip for the neonatal or even older children with the application of nasal cartilage distraction.更多还原