【作者】 屠军波； 杨壮群； 王正辉； 荔鹏； 虎小毅； 邢喆； 薛瑛；

【Author】 TU Jun - bo, YANG Zhuang - qun, WANG ZHeng - hui, LI Peng, HU Xiao - yi, XING ZHe, XUE Ying. Department of Oral and Maxillofacial Plastic Surgery, Stomatological College, Xi’ an Jiao Tong University, Xi’ an 710004

【机构】 西安交通大学口腔医学院口腔颌面整形外科；

【摘要】 <正>目的:构建游离毛囊体外培养模型,观察离体培养人头皮毛囊形态变化;观察神经生长因子(NCF)、雌激素(17β-E2)与米诺地尔对离体培养人头皮毛囊生长的影响。方法:在Williams E无血清培养基中,建立离体人头皮毛囊培养模型,组织学检测用冰冻切片光镜观察和电镜切片透射电镜观察;加入 NGF、雌激素和米诺地尔,通过目镜测微器测量毛囊长度,利用3H-TdR掺入检测毛囊24小时DNA合成率。结果:毛囊有不同程度的生长,随时间后移形态结构由清晰逐渐变为模糊;冰冻切片HE染色后在光学显微镜下可清楚分辨出毛囊的结构;培养3天毛囊的内外根鞘中可见凋亡细胞,内皮根鞘较外皮根鞘明显多见。NGF(100ng/ml)与米诺地尔(125ug/ml)对离体培养的人头皮毛囊的生长有促进作用(P<0．更多还原

【Abstract】 OBJECTIVE This study was carried out to construct the model of human hair follicle in vitro, observe morphologic change of the hair follicle and observe the effects of NGF, estrogens and minoxidil on the growth of human hair follicle in vitro. METHODS We established the model of human hair follicle in vitro in Williams E serum - free medium. .Histology form was observed by Cryo - section and transmission electron microscope. NGF, estrogens and minoxidil were added in. The length of hair follicle was measured by eyepiece micrometer and effects of NGF, estrogens and minoxidil to DNA synthesis rate of human hair follicle in vitro by measuring the rates of incorporation of 3H -TdR. RESULTS We found the human hair follicle in vitro have different degree elongation, whose constitution transform clouding followed by time. We can clearly tell the difference constitution of hair follicle under the light microscope after the Cryo - section was stained with HE. With transmission electron microscope observe hair follicle culture in vitro 3 days later, we found there are apoptosis cells in inner root sheath (IRS) and outer root sheath (ORS). Furthermore there are more apoptosis cells in IRS than in ORS. We found 100ng/ml NGF and 125ug/ml minoxidil respectively accelerating the growth of human hair follicle in vitro( P <0. 05). Whereas 0. 5ug/ml 17β - E2 inhibit the growth of human hair follicle in vitro( P <0. 05). When 100ng/ml NGF and 0. 5ug/ml 17β - E2 are added in medium together, there are no obviously changes on the growth of human hair follicle in vitro (P >0. 05). Result of measuring the rates of incorporation of 3H -TdR are accord with that of growth length. CONCLUSION When we culture human hair follicle in vitro, Williams E serum - free medium is a suitable choice. This model is a fine model also, which is used for screening drugs that may accelerate or inhibit the hair growth. Cryo - section can simplify procedur of paraffin section in histology detection of culture human hair follicle in vitro. We can observe the ultrastructural change of human hair follicle in vitro by transmission electron microscope, which can assist to observe apoptosis cells. The eyepiece micrometer is a simple tool for measuring length of hair follicle in vitro. Assisted by measuring the rates of incorporation of 3H -TdR, the RESULTS are further OBJECTIVE. 100ng/ml NGF and 125ug/ml minoxidil respectively accelerate the growth of human hair follicle in vitro. 0. 5ug/ml 17β - E2 can inhibit the growth of human hair follicle in vitro.100ng/ml NGF interfered in 0. 5ug/ml 17β - E2 inhibition growth of hair follicle in vitro.更多还原