【作者】 冯元勇； 陈万涛； 李宁毅； 周晓健； 蒋灿华； 叶冬霞；

【Author】 Yuanyong feng,Wantao Chen, Ningyi Li, Xiaojian Zhou, Canhua Jiang,Dongxia Ye Ninth People’s Hospital, Shanghai Second Medical University Medical College, Qingdao University,

【机构】 上海第二医科大学附属第九人民医院； 青岛大学医学院；

【摘要】 <正>目的:建立SD大鼠口腔颊粘膜鳞状上皮细胞癌细胞系。方法:采用4-硝基喹啉-1-氧化物(4NQO)自然饮水法36周诱发SD大鼠颊粘膜鳞状上皮细胞癌,无菌条件下取颊癌组织做组织块原代培养,细胞长出后呈各种细胞混合生长,主要表现为两大类细胞——上皮样细胞和成纤维样细胞。利用细胞体外培养更多还原

【Abstract】 Objective: To establish a cell line of squamous cell carcinoma(SCC) from Sprague-Dawley rat buccal mucosa. Methods: The Sprague-Dawley rats were induced with 4NQO water into animal model of buccal mucosa SCC. In vitro tissue and cell culture technique was employed to obtain mixed cells. The mixed cells were purified using trypsin digesting, scraping and cell cloning and the cells were cultured passage by passage,in vitro. The biological feature and cell growth of the cells were studied through pathological and immunohistochemical observation, chromosome analysis, cell number counting, flow cytometry assay and xenograft inoculation in nude mice. Result: The cultured cells were proved to be of squamous cell carcinoma cells. The cells have been cultured for 7 months, and transferred for over 100 passages. The population doubling period is 30 hours. The S-phase cells are 17.83%-22.13% in the cells. The rates of xenograft inoculation and lung planting by tail vein injection of carcinoma cells in nude mouse were both 100%. We have established a cell line which obtained from Sprague-Dawley rat buccal mucosa carcinoma. The cell line was named Sprague-Dawley rat buccal squamous cell carcinoma (SDSCC-B ).Conclusion: SDSCC-B is an oral squamous cell carcinoma cell line from Sprague-Dawley rat buccal mucosa.更多还原