【作者】 房思炼； 王大章； 郑光勇； 杨西川； 何志秀； 张杰；

【Author】 Fang Silian Wang Dazhang, Yang Xichuang, et al Dept of OMFS, College of Stomatology, West China University of Medical Science Now doing the postdoctoral research in Dept. of OMFS, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen Univeristy of Medical Sciences

【机构】 华西医科大学口腔医院口腔颌面外科； 华西医科大学口腔医院病理科； 华西医科大学附一院电镜室；

【摘要】 <正>目的:对抗VEGF单抗E11组、生理盐水组的颊癌组织进行形态学观察并作比较,以探讨E11 产生抑癌效应的机制,为临床应用E11提供依据。方法:接种人颊鳞癌的BALB／c裸小鼠分别通过腹腔或瘤周皮下注射抗人VEGF单抗和或生理盐水。接种后第18天处死,取颊癌组织进行光镜及更多还原

【Abstract】 Objective: The transplanted buccal carcinoma administrated anti-human VEGF mAb En or saline were observed by the pathological research. Based on the result, the mechanism of inhibition tumor was discussed. Methods: En was administered i.p. or. s.c. into BALB/c nu/nu mice which were transplanted buccal carcinoma. The saline administrated as negative control. Mice were killed on days 18 and the pathological alteration of tumor was evaluated. Results: Microscopically, massive necrosis and decreased vascular density was observed in tumor tissues treated by E11.Correspondent with that, the ultra-structure of tumor cells were degeneration and apoptosis. For the first time, degeneration and destruction of the vascular endothelial cell in the tumor tissues treated by En were observed by microscopy and transmission electron microscopy examination. Conclusion: Our study demonstrated that E11 inhibited the tumor angiogenesis by blocked the action of VEGF and resulted a significant inhibitory effect no tumor growth. The result would offer a therapeutic base to the clinical application anti-human VEGF mAb.更多还原