【作者】 郭传瑸； 张晔； 崔念辉； 俞光岩； 刘鼎新； 孟新聪； 宋青；

【Author】 Guo Chuanbin Cui Nianhui Yu Cuangyan et al Peking University School of Stomatology

【机构】 北京医科大学口腔医学院口腔颌面外科； 北京医科大学基础医学院细胞生物教研室；

【摘要】 <正>目的:了解变蓝菌素对口腔鳞癌及口腔粘膜内源性脂肪酸合成活性的抑制特点,并探讨其能否引发癌组织细胞凋亡。方法:切取口腔鳞癌患者新鲜手术标本的癌组织及正常口腔粘膜组织块, 分变蓝菌素处理与对照组。用[U-14C]乙酸钠示踪,液体闪烁计数14C的掺入量,以显示脂肪酸合更多还原

【Abstract】 Objective: Inhibition of cerulenin on the endogenous fatty acid synthetic activities of squamous cell carcinoma (SCC) and normal oral mucosa were assayed. Methods: Squamous cell carcinoma and normal oral mucosa were collected fresh from surgical specimens. The collected tissues were mined in RPMI and were divided into three groups: ceruleinin-treated, DMSO-treated, and control. The tissues were incubated in [U-14C] acetic acid, sodium salt for the last 2.5 hours of the treatment at 37℃ in 5% CO2. After labeling, total lipids were extracted and counted for 14C by scintillation counting. Results: Endogenous fatty acid synthetic activities of SCC in the cerulenin-treated group decreased by 19% at 1h, 64% at 2h, and 87% at 4h. It increased slightly the control group. The oral mucosa tissues were only mildly affected by cerulenin in fatty acid synthesis. Conclusion: Cerulenin significantly inhibits fatty acid synthetic activity in squamous carcinoma and only mildly affected the oral mucosa, indicating that the fatty acid synthetic path may be exploited as anticancer target.更多还原