【作者】 司晓辉； 杨连君；

【机构】 上海第二医科大学第九人民医院口腔医学院研究所； 第四军医大学病理学教研室；

【摘要】 <正>目的:分析细胞周期相关蛋白在颌骨骨肉瘤的表达及意义。方法:利用免疫组化ABC法对 cyclinD1,CDK4,E2F-1和Ets-1在20例颌骨骨肉瘤和8例软骨瘤的表达进行半定量检测。结果: 骨肉瘤cyclinD1,CDK4,p27,E2F01和Ets-1的阳性表达率分别为65(13／20),60％(12／20),20％(4／20), 70％(14／20)和5％(11／20),这些蛋白的表达在各病理分型间无显著性差异(P>0．05)。颌骨骨软骨瘤更多还原

【Abstract】 Objective: To analyze the expression of cell cycle-associated proteins in osteosarcoma of the jaws. Methods: Cyclin D1, CDK4, p27, E2F-1 and Est-1 expression were examined semi-quantitatively in 20 osteosarcoma and 8 osteocheondroma of the jaws by immunohistochemical ABC method. Results: The results demonstrated that cyclin D1, CDK4, 27, E2F-1 and Est-1 were positive 65% (13 of 20), 60%(12 of 20) ,20%(4 of 20), 70% (14 of 20 ) and 55% (11 of 20) in osteosarcoma of the jaws, respectively. There was no remarkable difference in the expression of these proteins among histopathologial types (P<0.05). In osteochondroma of the jaws, CDK4 and E2F-1 with an equal positivity of 12.5% (1 of 8), whereas p27 was positive 75%(6 of 8). None of osteochondroma was positive for cyclin D1 and Est-1. The positive rate of cyclin D1, CDK4, E2F-1 and Est-1 was significantly higher and p27 lower in osteosarcoma than that in osteochondroma of the jaws (P<0.05). In addition, the metastatic osteosarcoma of the jaws had higher positive rate of Est-1 than that of the non-metastatic (P<0.05). Conclusion: The results suggest that the dysregulation of cell cycle-associnated proteins may be involved in the occurrence and development of ostosarcma of the jaws.更多还原