【作者】 王松华； 田亚平；

【Author】 Wang Songhua Tian Yaping (The Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnaa University,Wuxi,214122)

【机构】 江南大学工业生物技术教育部重点实验室；

【摘要】 利用传统微生物筛选方法,从动物粪便中经过酸性平板初筛、中性平板复筛,得到一株产酸性脲酶的菌株JN_R12。通过比较形态特征、生理生化以及16S rDNA测序结果,结合系统发育分析,确定该菌为肠出血性大肠埃希氏菌Enterohemorrhagio Eschedchia coli O157:H7。JN_R12所产脲酶对酒精有较好的耐受性。离心后得到的全细胞在模拟酒样中的尿素去除率接近100%;黄酒中24 h孵化后的去除率在60%以上。更多还原

【Abstract】 A strain with acid urease catalytic activity,named JN_R12,was isolated from animal dropping using traditional acid and neutral pH plate screen.Based on the polyphasic study, including its phenotypio,physiological-biochemical properties,16S rDNA sequence similarity and phylogenetic analysis by using 16S rDNA sequencing,JN_R12 was identified as Enterohemorrhagic E.coli O157:H7.The urease of JN_R12 showed an incredible tolerance to alcohol,urea elimination ratio of centrifugal cells in simulation wine reached almost 100% in 3 days.When applying its urease to Chinese rice wine,up to 60% of the urea was eliminated after 24 h incubation.更多还原