Identification and prokaryotic expression of nuclear prelamin A recognition factor

【作者】 陈维春； 刘新光；

【Author】 Chen Weichun Liu Zhenjie Zhao Shuyong Zheng Huiling Liu Xinguang Insititute of aging,Guangdong Medical College,Dongguan 523808

【机构】 Insititute of aging,Guangdong Medical College；

【摘要】 <正>Nuclear prelamin A recognition factor,Narf,was identified by screening a human skeletal muscle library in a yeast two- hybrid interacting screen,which sequence was in agreement with narf from HeLa cells.Narf gene was obtained by PCR and cloned into prokaryotic expression vector pGEX-4T-1,generating the recombinant vector pGEX-Narf.BL (DE3) transfected with pGEX-Narf was inducted 5h by IPTG.A weak predicted 86-kD-band,the recombinant protein,and a 24- kD-degradation-band were showed by SDS-PAGE indicating that degradation of the recombinant protein was occurred, which laid a foundation for further function analysis of Narf.更多还原

【Abstract】 Nuclear prelamin A recognition factor,Narf,was identified by screening a human skeletal muscle library in a yeast two- hybrid interacting screen,which sequence was in agreement with narf from HeLa cells.Narf gene was obtained by PCR and cloned into prokaryotic expression vector pGEX-4T-1,generating the recombinant vector pGEX-Narf.BL (DE3) transfected with pGEX-Narf was inducted 5h by IPTG.A weak predicted 86-kD-band,the recombinant protein,and a 24- kD-degradation-band were showed by SDS-PAGE indicating that degradation of the recombinant protein was occurred, which laid a foundation for further function analysis of Narf.更多还原