【作者】 黄骏麒； 龚蓁蓁； 吴敬音； 陈松； 束春娥； 谢伟军； 周宝良； 沈新莲；

【Author】 Huang Jun-qi Gong Zhen-zhen Wu Jing-yin Chen Song Shu Chun-eXie Wei-jun Zhou Bao-liang Shen Xin-lian(Jiangsu Academy of Agricultural Sciences, Nanjing 210014;Shanghai Institute of Biochemistry and cell Biology, Academy Sinica, Shanghai 200031)

【机构】 江苏省农业科学院； 中国科学院上海生物化学细胞生物学研究所；

【摘要】 通过花粉管通道技术将慈姑蛋白酶抑制剂(arrowhead proteinase inhibitor,API)基因转入棉花,获得了转基因植株。经对棉铃虫抗虫性鉴定和PCR、RT-PCR、Southern blotting分析,证明获得了表达API基因的抗虫棉。这是迄今为止第一篇关于转API基因抗虫棉的报道。更多还原

【Abstract】 The gene for arrowhead proteinase inhibitor (API) was transferred into cotton plants by the pollen-tube pathway method, and transgenic cotton plant was resulted. After tests of resistance to pest insects and analyses of PCR, RT-PCR and Southern blotting, the transgenic plants were proved to have expressed the API gene and to be resistant to cotton bollworm. This is the first report so far concerning the pest-resistant transgenic cotton with the API gene.更多还原