【作者】 方孝东； 黄薇； 李冠一； 林栖凤；

【Author】 Fang Xiao-dong Huang Wei Li Guan-yi Lin Qi-feng(Institute of Biotechnology, Hainan University; Hainan Provincial Key Labof Biotechnology for Salt-tolerance Crops, Haikou 570228)

【机构】 海南大学生物科学技术研究所海南省耐盐作物生物技术重点实验室；

【摘要】 采用cDNA RDA技术,对盐生植物盐藻(Dunaliella salina)和秋茄(Kandelia candel)在盐胁迫时差异表达的基因进行了分离鉴定。由盐藻中共分离检测出10 个盐胁迫时差异表达的基因片段,其中5个与已知基因同源,且都与细胞分裂或盐胁迫有关,还有5个未知功能基因片段则是首次在盐藻中得到分离,对于盐藻耐盐可能具有重要意义。对其中的一个盐诱导表达基因片段进一步深入研究发现,盐藻线粒体DNA在盐处理过程中可能发生了扩增。据此推断,本实验条件下盐藻对抗盐胁迫的一个重要途径可能是通过降低细胞分裂频率来减少不必要的能量消耗,同时增加呼吸代谢的速率,产生更多的能量,以满足细胞对抗盐胁迫时对能量的需求。由秋茄中共分离检测出10个盐胁迫时差异表达的基因片段, 其中8个与已知基因同源,还有2个未知功能基因片段则是首次从秋茄中被分离,为深入了解秋茄耐盐机制,开发利用秋茄耐盐基因奠定了基础。更多还原

【Abstract】 Representational difference analysis of cDNA (cDNA RDA) has been performed to isolate the genes involved in salt-adaptation of two kind of Halophyte, Dunaliella salina and Kandelia candel. 10 differentially expressed genes were isolated from D. salina. 5 sequences were homologue to known genes of other organisms, either cell proliferation related or salt-tolerance related. The other 5 sequences with unknown function were identified from D. salina for the first time, and might be of importance for salt tolerance of D. salina. Further study on one of the isolated D. salina genes showed that mitochondrial DNA of D. salina might was amplified under salt stress. These results suggested that, under the condition of our experiment, an important strategy of D. salina to conquer salt stress might be lower the rate of cell division to lower unnecessary energy consumption, at the same time, increase the rate of respiratory metabolism to generate more energy to meet the increased energy requirement for cell to overcome salt stress. 10 differentially expressed genes were isolated from K. candel 8 sequences were homologue to known genes of other organisms; the other 2 sequences with unknown function were identified from K. candel for the first time. These results might help to understand the molecular mechanisms of salt tolerance of K. candel.更多还原