【作者】 李瑞生； 陈振文； 宋德光； 任文陟； 栾蓉晖；

【Author】 LI Ruisheng CHEN Zhzenwen SONG Deguang REN Wenzhi LUAN Ronghui (Laboratory Animal Center, Academy of Military Medical Sciences Beijing 100071 China)

【机构】 军事医学科学院实验动物中心； 解放军军需大学实验动物中心；

【摘要】 目的 本实验为了确立一种对近交系大鼠遗传物质的准确、简便的微卫星检测方法.方法利用聚合酶链反应(PCR)扩增技术对国内北京和哈尔滨等4家单位6个品系(SHR、SHRSP、LEW、RCS、WKY利F344)的8个近交系大鼠群体进行了DNA多态性的分析研究.结果 9个微卫星位点具有显著多态性:不同品系个体之间具有多态性;同一群体不同个体之间除SHR(哈)的SMST位点和WKY(哈)的AGT位点出现一定的差异外,其他均没有差异:不同地区同一品系不同个体之间也存在一定的差异.结论 该方法能有效地对近交系与杂交系、品系与品系、品系与亚系加以区分.因此,本实验为开展近交系大鼠遗传作图、基因定位和实验动物的遗传背景的监测提供可靠的信息,为大鼠遗传基因的研究提供了一个快捷简便、特异准确的方法.更多还原

【Abstract】 Objective In order to set up the exact, convenient method of PCR-analyzed microsatellites in the heredity monitoring of inbred rat. Method DNA polymorphism of 8 colonies from 6 inbred rat strains (SHR, SHRSP, WKY, LEW, RCS, F344) were studied in national Beijing and Haerbin using PCR-analyzed microsatellites. Results There were more polymorphism in 9 microsatellite loci; There is a polymorphism among the various rat strains; and no polymorphism in the same rat strains except SMST locus of SHR (Ha) and ACT locus of WKY (Ha) and there is a little difference from the same inbred rat strains in different areas. Conclusion The method of PCR-analyzed microsatellites can be used for distinguishing between inbred and outbred, different strains, strain and substrain. and it provides a lot of information for genetic mapping, gene location and heredity probe of the inbred rat strains, and a speedy, convenient method for genetic research of the inbred rat in the experiment.更多还原