【作者】 杨果杰； 陈秀莲； 邵萍；

【Author】 YANGCuoJie, et al (Department of Laboratory Animal Science, Health Science Center, Peking University, Beijing 100083, China)

【机构】 北京大学医学部实验动物科学部； 首都医科大学实验动物科学部；

【摘要】 本实验在能控制温湿度的实验室内进行,采用静脉注射不同剂量(0.5ng/kg、2ng/kg、20ng/kg、100ng/kg、200ng/kg、2000ng/kg)、大肠埃希菌内毒素(Lipopolysaccharied,LPS)的方法诱发免发热反应.结果表明:随LPS注射剂量的增加兔发热反应增强.表现在发热潜伏期逐渐缩短;发热高峰值逐渐增加;发热时程逐渐延长;体温反应指数逐渐加大.另外LPS注射剂量不同,动物发热曲线的峰型亦不相同,小剂量LPS(0.5ng/kg及2ng/kg)兔的发热反应呈单峰型;LPS用量增加到20ng/kg以上时,家兔发热反应呈双峰型.静脉注射剂量为100ng/kg～200ng/kg时兔发热反应呈典型的第二峰大于第一峰的双峰热,第一峰的高峰出现于注药后1.5h,第二峰的高峰出现于注药后3.5h,且发热反应均一,是造模的适宜剂量.实验室的温湿度、动物 的固定方法和动物的生理机能状态对实验结果也有影响.实验结果证明实验室的温度为25℃左右以颈部固定方法为宜.更多还原

【Abstract】 Intravenous application of endotoxin (ET) is a traditonal method to construct an animal fever model. The key factor is to find the optimum dosage.The fever was studied after different doses of ET were intravenously injected (Ⅳ) . The results of the study showed: 1) The increase of LPS dosage had a promotive effect on the febrile response of the rabbits with shorter latency to onset, higher peak fever change, longer time course and greater temperature response index (TRI) .2) The rabbits developed a positive dose - dependent fever in response to ET over the range of 0.5ng/kg-2000ng/kg.Small doses (0.5ng/kg, 2ng/kg) of ET induced a monophasic fever and large doses (20ng/kg, 100ng/kg, 200ng/kg, 2000ng/kg) of ET induced a biphasic fever. In response to the doses of 100ng/kg and 200ng/kg the rabbits developed a homogeneously typical biphasic fever.Therefore, the recommended range of ET dose is from 100ng/kg to 200ng/kg in making a rabbit fever model. The temperature and humidity of the ambient, rabbits restraining conditions and physical functions can also affect the experiment results.The results show that 25±1℃ ambient temperature is better than 18±1℃ and the neck stock is better than the prone stock.更多还原