【作者】 任欢； 邢淑贤； 徐红薇； 宋英晖； 商晓舟； 周贵生； 田景先； 李殿俊；

【Author】 REN Huan,XING Shu-xian,XU Hong-wei, et al (Department of Immunology, Harbin Medical University, Harbin 150086)

【机构】 哈尔滨医科大学免疫学教研室；

【摘要】 目的动态观察CIK(cytokine induced killer)细胞的体外增殖,体外的细胞毒活性,及通过动物实验研究其体内的抗肿瘤作用。方法通过提取健康供血者的PBMC,第0天加入γ-IFN,第1天加入IL-2、抗-CD3单抗和IL-1 培养CIK细胞;在流式细胞仪上做动态培养物的表型分析;与LAK细胞作对比,分别用MTT法测定其体外细胞毒活性及对S180荷瘤鼠的体内抗肿瘤作用。结果 CIK细胞在培养2周后获得大量增殖,表型分析表明,CIK细胞属异质性细胞群,在培养的过程中,群体的CD3+CD56+细胞大量扩增表达1000多倍,是CIK细胞的主要效应细胞;实验证明,CIK细胞的体外细胞毒活性及对S180荷瘤鼠的体内抗肿瘤作用均强于LAK细胞;其较强的体内抗瘤活性可能与荷瘤鼠主体内T细胞活化有关。结论 CIK细胞是一强于LAK细胞的、新型、高效、具有广谱杀瘤活力的免疫活性细胞。更多还原

【Abstract】 Objective To investigate the proliferation profile and the cytotoxicity of CIK( cytoline induced kill- er) cells, in vitro,and their anti-tumor effects on S180 bearing mice in vitro.Methods CIK cells were generated by culturing PBMC in the presence of γ-IFN on day 0 and IL-2,anti-CD3 McAb,IL-1 on day 1; CIK cultures were analyzed on different time points by FACS; Compared with LAK cells, the cytotoxicity in vitro by MTT assays and the anti-tumor effects on S180 bering mice in vivo of CDC cells were tested resecively. Results The flow cytometry analysis showed that CD3+ CD56+ T cells which were the major cytolytic effectors in CIK cells expanded 1 000-fold after 2 weeks in CIK culture;CIK cells had greater cytotoxicity against tumor cells in vitro than that of LAK cells; and had also significantly antitumor effects in mice bearing S180 tumor compared with LAK cells in vivo. (median inhibitory rates 78% vs 33% , P < 0.05); the CIK therapy was possibly correlated with induction of the activation of the splenic T lymphocytes of S180 bearing mice. Conclusion CDC cells were highly effcient cytolytic effector cells which were non-MHC restricted.更多还原