【作者】 谷鸿喜； 凌虹； 张凤民； 钟照华； 王文阁； 郭淑元； 娄阁； 郭彩玲；

【Author】 GU Hong-xi,LING Hong,ZHANG Feng-mini et al (Department of Microbiology, Harbin Medical University, Harbin 150086)

【机构】 哈尔滨医科大学； 中国军事医学科学院； 哈尔滨医科大学附属三院；

【摘要】 目的人乳头瘤病毒16型(HPV16)晚期基因(L1)编码病毒的主要衣壳蛋白,且HPV16感染与宫颈癌发生、发展关系密切,故对中国妇女感染的HPV16基因进行克隆及序列分析有重要实际意义。方法采用聚合酶链反应技术(PCR)扩增了3例中国妇女宫颈癌组织中HPV16的L1基因片段,并对其进行了克隆及全序列分析。结果发现3个HPV16L1基因核苷酸序列有4处均与最初报道的HPV16L1 DNA序列不同,并由此引起所编码的氨基酸亦发生变化。结论中国妇女宫颈癌组织中HPV16 L1核苷酸有一定程度变异。更多还原

【Abstract】 Objective Human papillimavirus type 16 (HPV16) is highly related with the development of cervical carcinoma. HPV16 late gene L1 encodes its main capsid protein. This study is to analyze the whole se- quence of L1 gene of HPV16 of the Chinese isolates. Methods Three samples of HPV16 L1 gene ere amplified from cervical carcinoma tissues of Chinese patients by PCR and then cloned and sequenced. Results there were four sites in nucleic acid sequences of all three HPV16 L1 fragments were different from the originally reported sequence of HPV16 and the differed sequences had changed the triplet codes, therefore, subse-quenely changed the amino acids it coded. Conclusion the results showed that some mutation had taken place in the nucleotide sequence of L1 gene of HPV16 obtained from the cervical carcinoma tissues of Chinese women.更多还原