【作者】 张凤蕴； 吕雪莹； 张飚； 王丽群； 周贵生； 赵育莹； 李殿俊；

【Author】 ZHANG Feng-yun,LI Xue-ying,ZHANG Biao.et al ( Department of Immunology, Harbin Medical University, Harbin 150086, China )

【机构】 哈尔滨医科大学免疫学教研室；

【摘要】 目的进一步探讨多发性硬化(MS)和实验性变态反应性脑脊髓炎(EAE)的发病机制。方法取EAE动物的脾细胞,制备淋巴细胞悬液,以荧光探针Fluo-3／AM负载后,通过激光扫描共聚集显微镜(Insight plus IQ,Meridi- an,USA)检测细胞荧光强度,其荧光强度与细胞内钙离子浓度成正比。结果 EAE组淋巴细胞内钙离子浓度明显高于正常对照组。结论细胞内钙在EAE动物淋巴细胞的过度活化方面发挥重要作用。更多还原

【Abstract】 Objective to investigate the implicated pathogenesis of multiple sclerosis and experimental allergic en-cephalomyelitis.Methods The Lymphocytes from guinea pig with experimental alleigic encephalomyelitis(EAE) were loaded with Flou-3/AM,a Ca2+ fluorescent indicator. The intracellular calcium concentration ([Ca2+ ];) was measured by using confocal microscope and [Ca2+ ]i changes were represented by fluorescent intensities.Results The [Ca2+ ]; was significandy increased in lymphocytes of guinea pig with ElAE compared with that of normal control group.Conclusion the [Ca2+ ]; played an important role in lymphocyte activation of EAE.更多还原