【作者】 冷卫东； 王大章； 冯戈； 何嘉；

【Author】 LENG Wei-dong (Department of stomatology,Taihe Hospital,Yunyang Medical college,Shiyan 442000,Hubei,China) WANG Da-zhang (Department of oral and maxillofacal surgery,West China School of Stomatology,Sichuan University,Chengdu 610041,China)

【机构】 湖北十堰市郧阳医学院附属太和医院口腔中心； 四川大学华西口腔医学院颌面外科；

【摘要】 目的探讨热放疗对舌鳞癌Tca 8113细胞和耐药的Tca 8113/CBDEA细胞耐药性的影响。方法实时定量逆转录PCR（real-time quantitative RT-PCR）检测热放疗对Tca 8113细胞和耐药的Tca 8113/CBDEA细胞多药耐药基因1（MDR₁）、多药耐药相关蛋白1基因（MRP₁）和谷胱甘肽硫-转移酶π基因（GST—π）表达的影响和检测热放疗对细胞内阿霉素浓度的影响。结果Tca 8113/CBDEA和Tca 8113细胞热放疗后耐药基因MDR₁的表达在4小时和24小时无明显改变（P>0.05）。MRP₁基因的表达在4小时和24小时组均有明显下降（P<0.05）,.GST-π基因表达在4小时组Tca 8113和Tca 81 13/CBDEA的表达无明显改变（P>0.05）,在24小时组有明显下降（P<0.05）.热放疗以后肿瘤细胞内阿霉素（ADM）浓度有明显上升。结论热放疗联合应用抑制了放疗造成的耐药基因表达上升,增加了细胞内的药物浓度,,热放疗联用不会促使肿瘤细胞产生MDR更多还原

【Abstract】 Objective To study effect of thermoradiotherapy on expressions of MDR genes in tongue squamous cell carcinoma cell line Tca 8113 and its multidrug resistance（MDR）cell line Tca8113/CBDEA and intracellular ADM concentration.Methers Cell lines were thermoradiotherapy-treated（42℃for 0.5h and 2Gy of radiotion）,4h and 24h later real-time quantitative reverse transcriptase-polymerase chain reaction（RT-PCR）detected expressions of MDR relative genes（MRP₁,MRP₁,GST-π）and HTS 7000 Plus Bio Assay Reader measured intracellular ADM concentration.Results Post thermoradiotherapy,detection of real time PCR showed there were not alteration in the expression of MDR₁ gene （P>0.05）..Expressions of MRP₁ gene significantly descended at 4h and 24h postthermoradiotherapy（P<0.05）..PCR showed there were not alteration in the expressions of GST-πgene at 4h postthermoradiotherapy in Tca 8113 and Tca 8113/CBDEA cell line（P>0.05）,but there was significantly decrease at 24h（P<0.05）. Drug tolerance decreased.hyperthermia increased intracellular drug concentration in Tca 8113/CBDEA and Tca 8113（P<0.01）...Conclusions thermoradiotherapy enhance chemotherapy effect and suppress the expressions of MDR genes induced by radiation. The combination of hyperthermia and radiotherapy does not induce MDR.更多还原