【作者】 李伟忠； 丁彦青； 李祖国； 王小燕；

【Author】 LI Weizhong~1 DING Yanqing~2 LI Zuguo~2 WANG Xiaoyan~2 Department of Stomatology1,Department of Pathology2,Nanfang Hospital,Southern Medical University,Guangzhou 510515

【机构】 南方医科大学口腔医学院口腔颌面外科； 南方医科大学口腔医学院病理科；

【摘要】 目的:研究选择性COX-2抑制剂Celecoxib对人舌鳞癌Tca8113细胞生长抑制及诱导凋亡的作用。方法:Tca8113细胞采用不同浓度的CeIecoxib 2.5,5,10,20,40,80μmol作用后24、48、72小时。采用MTT法测定细胞存活率,采用流式细胞仪测定不同浓度的celecoxib对Tca8113细胞增殖周期的改变。流式细胞仪annexin V检测、荧光显微镜及透射电镜观察细胞凋亡情况。结果:Celecoxib可显著抑制Tca8113细胞增殖,这种作用是剂量依赖性的,并且通过阻止G0/G1细胞进程抑制细胞周期进程,同时诱导细胞凋亡的发生。结论:研究认为,抑制COX-2酶的活性可能是治疗舌癌的有效措施之一。更多还原

【Abstract】 Purpose:To explore the effect of Celecoxib on the growth inhibition and inducing apoptosis on Tca8113 cell lines.Tca8113 cell lines were treated with celecoxib at different dosage.The cell viability and Apoptosis was evaluated after 24,48h of incubation with celecoxib (40,80μmol/L/well) by flow cytometry via annexin V assay,and with fluorescence microscope and TEM.The cell cycle was analysised with flow cytometry. Results:Celecoxib treatment resulted in significant antiproliferative effects on Tca8113 cell lines.Treatment of these cells with celecoxib dose-dependently,reduced cell growth with arrest of the cell cycle at G0/G1 phase and induction of apoptosis.Conclusions:Our findings suggest that COX-2 inhibition has potential to become an effec- tive treatment for human squamous cell carcinoma of tongue.更多还原