【作者】 刘来奎； 许小会； 陈盛； 宋晓陵；

【机构】 南京医科大学口腔医学研究所,南京医科大学口腔医学院口腔医学院基础教研室；

【摘要】 目的:观察经颧弓后上牵引下颌后兔颞下颌关节滑膜中PTHrPmRNA的分布特征,探讨PTHrP在颞下颌关节紊乱病滑膜病理改变中的意义。方法:手:术在颧弓和下颌角之间放置弹簧持续地将兔右侧下颌骨向后上方牵引,分别于术后2,4,6周处死动物,采用HE及原位杂交染色观察实验组与对照组关节滑膜的病理改变及其PTHrPmRNA的分布特征。结果:经颧弓后上牵引下颌早期(2周),关节滑膜表面绒毛状凸起,滑膜内层细胞增生明显,下层成纤维细胞增加,血管壁增厚;膜内层细胞及其下层成纤维细胞中可见PTHrPmRNA阳性表达。随牵引时间的延长,滑膜下组织出现新生血管,伴炎细胞浸润,部分动物关节滑膜萎缩发生纤维粘连,滑膜内层细胞及下层成纤维细胞PTHrPmRNA表达明显增强。对照组PTHrPmRNA在整个实验过程中均处于弱表达或不表达。结论:PTHrP可能通过自分泌/旁分泌途径参与颞下颌关节紊乱病滑膜病理改变,在颞下颌关节紊乱病骨及软骨改建中发挥重要作用。更多还原

【Abstract】 Objective:to investigate the roles of PTHrP in the synovium of temporomandibular joint after over mechanical loading.Methods:Fifteen adult New Zealand rabbits were subjected to trac- tion between the mandibular ramus and zygomatic arch in the postero-superior direction unilaterally u- sing elastic force.The rabbits were killed respectively at 2,4 and 6 weeks and the histopathologic change was observed through Hematoxylin & Eosin stain.The expression of PTHrP mRNA in the synovium of TMJ was observed by in situ hybridization staining.Results:Synovial membrane involvement in temporoman- dibular joint disorder is characterized by an early proliferative phase (2 weeks) with synovial intima hy- perplasia and cell hypertrophy,and subsequently in deposition of fibrous material in the intima ma- trix.The late phase is characterized by extensive fibrosis of the subintimal tissue.In joint synovium, PTHrP mRNA staining was detected in synovial intima and subintimal fibroblasts at the early stage after operation.Expression of PTHrP mRNA was obviously stronger in the four or six weeks after trac- tion.PTHrP staining was negative or weaker in control.Conclusion:These results are consistent with a possible paracrine pathway for PTHrP action in the synovial pathogenesis of temporomandibular joint dis- order.更多还原