【作者】 赵运军； 何利刚； 王会良； 许淼； 刘德春； 孙中海；

【Author】 ZHAO Yun-jun HE Li-gang WANG Hui-liang XU Miao LIU De-chun SUN Zhong-hai~* (Ministry of Education/National Center for Citrus Breeding,Huazhong Agricultural University,Wuhan 430070,China)

【机构】 华中农业大学国家柑橘育种中心；

【摘要】 CBF 基因是植物冷驯化信号转导途经中的一个关键基因,在低温、干旱诱导下表达,因此其启动子很可能是一个非生物逆境诱导启动子,而逆境诱导启动子在植物基因工程中起着重要作用。根据柑橘近缘植物枳壳(Poncirus trifoliata(L.)Raf.)的 CBF 类似基因 PtCBF1的 cDNA 设计引物克隆出其 DNA 序列,发现 PtCBF1不含内含子。利用 TAIL-PCR 及 SON-PCR 进行两轮染色体步行,克隆出 PtCBF1的启动子序列,并对该序列进行生物信息学分析,发现克隆的 PtCBF1启动子很可能是逆境诱导的启动子。更多还原

【Abstract】 CBF gene was a key gene in plant cold acclimation signal transduction pathway,which was induced by cold or drought stress,so its promoter was a putative abiotic stress induced one.And it’s known that stress-induced promoter played an important role in plant genetic engineering.In this work,primers,on the basis ofcDNA of PtCBF1,a CBF- like gene in the interfertile citrus relative,Poncirus trifoliata(L.)Baf.,were designed to clone its DNA.And it was discovered that PtCBF1 gene didn’t have intron.Then PtCBF1 promoter sequence was isolated via two step chromosome walking based on TAIL-PCR and SON-PCB.Bioinformatics analysis was performed,and it’s presumed that the promoter was likely to be stress-indueed.Thus this work laid a foundation for further promoter function analysis and plant genetic engineering expression.更多还原