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用抑制性消减杂交法分离兴春椪柑干旱胁迫诱导表达的cDNA
Isolation and cloning of cDNA induced by drought stress in ponkan(C.reticulata)using suppression subtractive hybridization
【Author】 TAN An-qun~(1a) ZHONG Guang-yuan~(2*) JING Dong~2 YUAN Hu-bin~(1a) WANG Mira~(1b) (1.a.College of Horticulture and Forestry;b.CoHege of Life Science,Southwest University,chongqing 400716,China; 2.The Citrus Research Institute,Chinese Academy of Agricultural Sciences,Chongqing400712,China)
【机构】 西南大学园艺园林学院； 中国农业科学院柑橘研究所； 西南大学生命科学学院；
【摘要】 对兴春椪柑进行干旱胁迫,以其干旱胁迫叶 cDNA 为试验方(tester),正常生长的兴春椪柑叶 cDNA 为驱动方(driver),利用抑制性消减杂交技术(SSH)构建了干旱胁迫下兴春椪柑的消减文库。重组克隆经 PCR 检测,插入片段大部分集中在250～700 bp 之间.通过对文库阳性克隆随机挑取85个测序,成功获得79条 EST,获得非重复 EST 58条.
【Abstract】 A suppression subtractive hybridization library of Ponkan leaves(C.reticulata)was constructed by using the drought treated leaves as tester and leaves grown under normal conditions as driver.The PCR results of recombinant clones showed that the inserts were mainly 250～700 bp.A total of 85 positive clones were randomly picked out from the subtracted library and sequenced,a total of 79 positive clones were obtained,and 58 unique ESTs were gained bassed on the sequening data.
- 【会议录名称】 中国柑橘科技创新与产业发展战略论坛暨中国柑橘学会2007年年会论文集
- 【会议名称】中国柑橘科技创新与产业发展战略论坛暨中国柑橘学会2007年年会
- 【会议时间】2007-11
- 【会议地点】中国湖南长沙
- 【分类号】S666.1
- 【主办单位】湖南农业大学学报