【作者】 王天奇； 董发明； 张玲； 郭巍； 杨光忠；

【Author】 Wang Tian-qi Dong Fa-ming Zhang Ling Guo Wei Yang Guang-zhong (Livestock Science and Technology College,Henan University of Science and Technology,Luoyang 471003,China)

【机构】 河南科技大学动物科技学院；

【摘要】 试验观察了结肠小袋纤毛虫(Balantidium coli)在体外培养条件下的生长情况。结果表明,结肠小袋纤毛虫滋养体在体外的最适生存温度为28℃,在洛克氏液中生存时间明显长于生理盐水和蒸馏水,在RSS培养基及双相培养基中可在培养24h时达到高峰。在含有诺氟沙星的兔血水培养基中,滋养体的数量可在培养后96h达到高峰,生存时间达成180h。更多还原

【Abstract】 The conditions for cultivation of B.coli in vitro were investigated in the present study.Results showed that the optimum temperature for cultivation of B.coli in vitro was 28℃,the surival time of trophozoites of B.coli in Ringer was longer than that in normal saline and in distilled water,the number of trophozoites of B.coli reached a high peak at 24h after cultivation in RSS medium and in double medium.In the medium which contain rabbit blood,distilled water,Norfloxacin,the number of trophozoites of B.coli reached a high peak at 96h after cultivation,and the survival time of B.coli approached up to 180h.更多还原