【作者】 李奎； 李建华； 方丽云； 王超； 刘英； 康相涛；

【Author】 Li Kui~(1,2) Li Jian-hua~1 Fang Li-yun~1 Wang Chao~3 Liu Ying~2 Kang Xiang-tao~(1,4) (1.College of Animal Husbandry and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002; 2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070; 3.Office of Animal Husbandry in Yuanyang County of Henan Province,Yuanyang 453500; 4.Study Centre on Poultry-breed Resources and Original Project of Henan Province,Zhengzhou 450002)

【机构】 河南农业大学牧医工程学院； 甘肃农业大学动物医学院； 河南省原阳县畜牧局； 河南省家禽种质资源创新工程研究中心；

【摘要】 【研究目的】探讨细胞凋亡基因p53在固始鸡免疫器官内的表达及其生理意义:【方法】应用免疫组织化学技术和Leica显微图像处理系统:【结果】p53阳性蛋白表达于法氏囊和胸腺内淋巴细胞细胞膜、细胞质和细胞核。在不同发育阶段固始鸡法氏囊内均有少量p53表达阳性细胞,阳性细胞主要分布于黏膜上皮层与淋巴小结之间靠近上皮层的固有膜内,其次是淋巴小结边缘及淋巴小结与淋巴小结之间的固有膜层内。在1周龄和12周龄固始鸡胸腺内有少量p53表达阳性细胞,阳性细胞主要分布于胸腺小叶皮质与髓质交界处,其次是皮质内,很少出现在髓质内。p53在不同发育阶段固始鸡脾脏内均无表达:【结论】在正常生理情况下,细胞凋亡基因p53参与了固始鸡中枢免疫器官内淋巴细胞发育分化过程中的凋亡调控,对免疫器官的稳定发挥重要作用。更多还原

【Abstract】 【Objective】The expression of the apoptotic gene-p53 in immunity organs of Gushi chickens was observed and its physiological significane was discussed;【Method】By using immunohistochemistry technology and Leica Microsystems;【Results】The positive p53-protein was found in cell membrane,cytoplasm and nucleus of thymocytes and lymphocytes,p53 couldn’t expressed in spleen of Gushi chickens normally,but expressed in thymus and Bursa.The majority of positive cells,in Bursa of Gushi chickens,were observed in lamina propria of mucous membrane and areas between lymphoid nodules,a few in edge of lymphoid nodules,and few in inner of lymphoid nodules.A few of p53 positive cells distributed in the connective zone of thymic cortex and thymic medulla,secondly in thymic cortex,and few in thymic medulla for 1-week and 12-week Gushi chickens;【Conclusion】All results mentioned above indicated that p53 participated in the regulation of apoptosis of thymocytes and lymphocytes in central immunity organs of Gushi chickens.It is important for the stability of immunity organs in Gushi chickens.更多还原