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野生稻转育品系挥发性提取物对褐飞虱的影响

Attractive response of brown planthopper to the volatile extraction of introgressive lines from wild rice

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【作者】 郑浩杨长举尹业平江洪

【Author】 Zheng Hao~1 Yang Changju~1 Yin Yeping~2 Jiang Hong~2 (1.Department of Plant Protection,Huazhong Agricultural University,Wuhan 430070;2.College of Science,Huazhong Agricultural University,Wuhan 430070)

【机构】 华中农业大学植物保护系华中农业大学理学院

【摘要】 利用 SDE 法提取3种水稻的挥发性物质,用浓缩后的提取物作选择反应实验。在6个记录时间中,以第15min 响应率最高,试虫在包括15min 在内的连续 5个记录时间的相应没有显著差异。在响应时间上,前5min,B12与 TN1类似。随后 B12响应率下降,以 TN1响应率最高,B12最低。稻褐飞虱对各品系提取物的响应以 TN1趋向性,B12其次,B5最低。而稻褐飞虱在各品系提取物间的选择与其对各品系植株的响应略有不同。试虫在 B5/B12的选择中,完全不选择 B5。由此表明,TN1挥发物中对褐飞虱的引诱因子强于 B5及 B12,其中 B5的引诱因子极少。B5挥发物中有对褐飞虱强烈的驱避因子,褐飞虱对 B12挥发物有一定的忍耐能力。

【Abstract】 The stream distillate of three varieties,TN1 (susceptible),B12 (moderate resis- tant) and B5 (high resistant) were prepared by using simultaneous distillation-extraction (SDE),followed by the condensation by K-D condenser with the headspace protection of nitro- gen at the room temperature.The time-sequence experiment was conducted to screen the time that most stable and comfortable for BPH’s tendency to stream distillate.30 minutes was broken down into 6 parts with each equal and the tendency rate was recorded at each shift,shows that the most stable time for recording BPH’s tendency is at the 15 minute. The choice-test between the distillate of each line and acetone (as control) shows that: 1) the preference of BPH to the line was ordered descendingly as TN1,B12 and B5 with sig- nificant difference between each;2) the tendency to control was ordered descendingly as B5, TN1,B12,but without significant difference between CK in the combination CK/TN1 and that in the CK/B12,suggesting some possible repellents are existed in the B5.

【关键词】 野生稻转育品系挥发物褐飞虱
【Key words】 wild riceintrogressive linevolatilebrown planthopper
【基金】 国家自然科学基金;湖北省自然科学基金
  • 【会议录名称】 华中昆虫研究(第一卷)
  • 【会议时间】2002-09
  • 【分类号】S435.112.3
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