【作者】 傅冬和； 刘仲华； 黄建安； 陈惠衡；

【Author】 Fu Donghe~(1 2) Liu Zhonghua~(1 2*) Huang Jianan~(1 2) Chen Huiheng~2 (1 Tea Key Lab of the Ministry of National Teaching,2 College of Horticulture and landscape,Hunan Agricultural University,Hunan,Changsha 410128)

【机构】 湖南农业大学教育部茶学重点实验室； 湖南农业大学园艺园林学院；

【摘要】 将茯砖茶依次用三氯甲烷、乙酸乙酯及正丁醇萃取后制各样品,测定各部分样品对a-淀粉酶及胰脂肪酶活性的影响。研究发现茯砖茶各部分样品对胰脂肪酶活性的影响程度不同,三氯甲烷层稍有抑制作用,活性只有对照的0.82倍,水提物、乙酸乙酯萃取层及正丁醇萃取层有显著的激活作用,正丁醇萃取层部分激活作用最明显,达2.54倍。茯砖茶各部分样品对a-淀粉酶活性均有激活作用,其中以乙酸乙酯萃取层部位的激活作用最强,激活倍数为4.09,其次是水提物,其激活倍数为2.99,水层的激活作用不显著。三氯甲烷萃取层抑制作用不显著,活性只有对照的0.88倍。乙酸乙酯萃取层及正丁醇萃取层部分经过HP-20树脂再分离后,各部分仍有较高的活性。更多还原

【Abstract】 Fuzhuan Tea was extracted by chloroform,ethyl acetate,1-butanol in turn.The effect of Fuzhuan Tea on the activities of metabolize mnzyme was researched.The research results showed that different fractions of Fuzhuan Tea had different effects on pancreatic lipase activity.The sample extracted by chloroform could restrain pancreatic lipase a little and the multiple was 0.82.The water extracts,the sample extracted by ethyl acetate and 1-butanol had obvious activation,that of the sample extracted by 1-butanol was the most active.The multiple was 2.54.All fractions of Fuzhuan Tea were active to a-amylase,among them,the sample extracted by ethyl acetate was the most active.The multiple was 4.09. The water extracts was the second one and its multiple was 2.99.That of the layer of water sample was not obvious.The sample extracted by chloroform could restrain a-amylase a little and the multiple was 0.88.The samples separated from the sample extracted by ethyl acetate and the sample extracted by 1-butanol were active to a-amylase and pancreatic lipase respectively.更多还原