焦炉工外周血淋巴细胞DNA损伤和血清SOD活力的检测

【作者】 李时恩； 李春阳； 徐玉宝； 任丽敏； 刘琳琳； 吴逸明；

【机构】 郑州大学公共卫生学院劳动卫生与职业病学教研室；

【摘要】 目的:检测焦炉工外周血淋巴细胞DNA损伤和血清SOD活力,寻找职业性肺疾患高危人群的早期检测指标。方法:用单细胞凝胶电泳法对33名焦炉工和16名正常对照外周血淋巴细胞DNA损伤检测,用试剂盒对血清SOD活力进行检测。结果:接触组外周血淋巴细胞DNA损伤阳性率及损伤程度明显高于对照组(P<0.01)。结论:焦炉逸散物作业工人细胞DNA损伤增加,血清SOD活性下降,且接触不同浓度的焦炉逸散物SOD活力下降程度不同。更多还原

【Abstract】 Objective:To explore lymphocyte DNA breakup and serum reactive oxygen species(ROS) level in coke oven workers and to screen the earlier biomarkers for occupational pulmonary cancer.Methods:DNA breakup was detected using the method of single cell gel electrophoresis(SCGE) and ROS level were assayed by the method of Fenton in 33 coke oven workers and16 normal controls,respectively.Results:DNA breakup rate was markedly higher in coke oven workers than that of controls(P<0.01),and DNA damage rate increased with the work-age(P<0.05).The serum ROS level was also much higher in coke oven workers than that of controls(P<0.05).Conclusion:The workers exposed to coke oven volatiles could cause the increase of DNA damage rate and ROS level,and SOD activity declined to be different when exposed to different concentrations of coke oven volatiles access to different concentrations of coke oven dispersion of SOD activity declined to be different.更多还原