【作者】 陈晓杰； 薛乐勋； 丁兰平； 宫亚欧； 杨胜利；

【Author】 CHEN Xiao-jie XUE Le-xun DING Lan-ping GONGYa-ou YANG Sheng-li (Departement of Esophgeal Cancer Reaserch,zhengzhou university,zhengzhou Henan 450052)

【机构】 郑州大学医学院食管癌研究室；

【摘要】 背景与目的:探讨皂荚提取物对人食管鳞癌细胞株EC9706的生长抑制作用及其作用机制。材料与方法:用光学显微镜观察细胞形态学变化,采用MTT法检测不同浓度皂荚提取物对EC9706细胞增殖的影响;采用TUNEL法检测皂荚提取物对EC9706细胞凋亡的影响;采用Weston blotting检测皂荚提取物对EC9706细胞bcl-2表达的影响。结果:不同浓度皂荚提取物(100μg/ml-200μg/ml)对EC9706细胞的增殖有明显抑制作用(P<0.01),并具明显的浓度依赖效应(r=0.768);皂荚提取物可诱导EC9706细胞凋亡,抑制EC9706细胞Bcl-2蛋白表达。结论:皂荚提取物可抑制EC9706细胞的增殖,诱导细胞凋亡。该效应可能与皂荚提取物抑制细胞bcl-2的表达有关。更多还原

【Abstract】 Background and Aim:This study was designed to observe the growth inhibition of the extract from Chinese honeylocust fruit on esophageal squamous carcinoma cell line EC 9706. Materials and Methods:The chang of cell morphology is Observed by light microscope;MTT assay,TUNEL cell apoptosis detection and western blotting was used to get informations of EC9706 which was treated with the extract.Results:Different concentration(100μg/ml-200μg/ml) of the extract from Chinese honeylocust fruit remarkably inhibited the growth of EC9706 cells (P<0.01),and displys concentration depend effect(r=0.768).The extract could induce apoptosis and regulate the expression of bc1-2 protein in EC9706 cells.Conclusion:The extract from Chinese honeylocust fruit can inhibit the growth of EC9706 evidently.The extract can also induce the apoptosis of EC9706 cell and decrease the expression of bc1-2 in EC9706,and these may be one of the mechanisms that the extract inhibit the growth of EC9706.更多还原