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河蚌酶解降血压肽的初步分离及性质研究

Separation And Character of Anti-hypertensive Peptides Hydrolyzed from Mussel

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【作者】 戴志远朱凤仙张燕平袁晓晴

【Author】 Dai Zhiyuan Zhu Fengxian Zhang Yanping Yuan Xiaoqing (Institute of Aquatic Products Processing,Zhejiang Gongshang University,Hangzhou 310035)

【机构】 浙江工商大学水产品加工研究所

【摘要】 以酶解河蚌多肽为原料,对其中的降血压肽进行了初步分离及性质研究。采用凝胶Sephadex G-50对河蚌酶解多肽进行分离纯化得到两个组分MPP1和MPP2,分子量分别集中在14125Da和1271Da左右。用高效液相色谱(HPLC)进行血管紧张素转化酶(ACE)抑制率测定,其半抑制浓度(IC50)分别为0. 88mg/mL和0.32mg/mL。结果表明,组分MPP2具有明显的降血压作用。氨基酸组成分析结果表明, MPP2中谷氨酸(Glu)含量最高,达14.04g/100g,疏水性氨基酸含量高达29.76g/100g,与其较高的降血压活性有密切关系。

【Abstract】 The preliminary separation and characterization of anti-hypertensive peptide(s),enzymatically hydrolyzed from mussel proteins,were investigated.The fractions of MPP1 and MPP2 were obtained by gel chromatography on column of Sephadex G-50,with the molecular weight distribution of about 14125Da and 1271Da respectively.The IC50 of angiotensin converting enzyme was respectively 0.88mg/mL and 0.32mg/mL by the means of high performance liquid chromatography.The results showed MPP2 had stronger anti-hypertension effect than that of MPP1.Furthermore,the amino acid test manifested that the content of Glu in MPP2 was highest(14.04g/100g) and the total content of hydrophobic amino acids was 29.76g/100g.This was closely related with the high anti-hypertension effect.

  • 【会议录名称】 营养与食品——健康中国高级论坛Ⅱ论文集
  • 【会议名称】营养与食品——健康中国高级论坛Ⅱ
  • 【会议时间】2008-05
  • 【会议地点】中国辽宁大连
  • 【分类号】R151
  • 【主办单位】中国食品科学技术学会营养支持专业委员会、中国环境诱变剂学会膳食与疾病专业委员会
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