【作者】 唐小江； 马争； 黄建勋； 黄汉林； 李来玉； 牛侨；

【Author】 HUANG Jiangxun,MA Zheng TANG Xiaojiang,et al.(Guangdong Provincial Center for Occupational Disease Prevention and Treatment, Guangzhou,510300,China)

【机构】 广东省职业病防治院； 山西医科大学；

【摘要】 目的研究正己烷慢性中毒对红细胞膜血影蛋白(Spectrin,Sp)交联的影响。方法SD大鼠灌胃给予正己烷0、168、675、2700 mg/kg,连续8周。抽提红细胞膜蛋白,用双缩脲法测定蛋白浓度,用聚丙烯酰胺凝胶电泳(SDS-PAGE)后以硝酸银染色,光密度扫描计算蛋白含量,用Western blot鉴定血影蛋白。结果染毒8w后,2700mg/kg组动物,红细胞膜蛋白浓度为:5.88±0.89g/L,经SDS-PAGE,硝酸银染色见410kD处有异常蛋白条带,经Western blot证实为Sp共价交联产物,带形为连续"拖尾"状。共价交联部分占总Sp的百分比呈时间-剂量-效应关系。结论正己烷可引起红细胞膜血影蛋白交联。更多还原

【Abstract】 Objective To study the cross-linking of erythrocyte spectrin caused by n-hexance chronic poisoning.Method SD rats were given n-hexane of 0、168、675、2700 mg/kg for 8 weeks(5 d/w) ig.8 weeks later,protein was eluted from erythrocytes and then the concentration was tested.It was isolated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),dyed by silver staining,quantified by scanner and identified by Western blot.Results In the group of 2700mg/kg,at the end of the test(8 weeks after the test started),the concentration erythrocytes protein of was 5.88±0.89g/L.A high-molecular-weight protein band for about 410 kD was showed on SDS-PAGE in the rats of 2700 mg/kg.It was identified by Western blot as the covalent cross-linking spectrin(α,β-heterodimer) with a long"tailling".There were time-dose-effect relationship with the increase of cross-linking spectrin.Conclusion n-Hexane could mediated erythrocyte spectrin covalent cross-linking更多还原