【作者】 项翠琴； 张云英； 秦逸秋； 沈春琳； 刘茶珍； 陈纪刚； 张胜年；

【Author】 XIANG Cui-qin,ZHANG Yun-ying,QIN Yi-qiu,SHEN Chun-lin,LIU Cha-zhen,CHEN Ji-gang,ZHANG Sheng-nian.*Shanghai Municipal Center for Disease Control & Prevention,Shanghai 200336,China

【机构】 上海市疾病预防控制中心； 复旦大学公共卫生学院；

【摘要】 目的研究职业性联苯胺接触者和膀胱癌患者突变型p53蛋白的表达。方法用免疫-PCR法测定职业性联苯胺接触人群和膀胱癌患者血中突变型p53蛋白,用巴氏分级法对尿脱落细胞进行分级。结果职业性联苯胺接触接触人群中,突变型p53蛋白的阳性率随着巴氏级别的升高而增加;膀胱癌现患病例组中p53突变型蛋白的阳性率显著高于职业性联苯胺接触人群。p53突变型蛋白PCR扩增条带经扫描积分后,巴氏级别为2、2.5、3级的三组人群、有膀胱癌病史组以及膀胱癌现患病例组中的平均积分均显著高于巴氏级别1级组。结论膀胱癌患者血中p53突变型蛋白有较高的检出率,可成为膀胱癌诊断的辅助指标。更多还原

【Abstract】 Objective To study expression of mutated p53 protein in workers occupationally exposed to benzidine and bladder cancer patients.Methods Mutated p53 protein in serum was determined using Immuno-PCR in workers occupationally exposed to benzidine and bladder cancer patients,while exfoliated urothelial cells were classified with Papanicolau method.Results Positive rate of mutated p53 protein increased with Papanicolau level in workers occupationally exposed to benzidine;the positive rate of mutated p53 protein in bladder cancer patients was significantly higher than that in workers occupationally exposed to benzidine.The scanning integrals at PCR amplified bar of Papanicolau level 2,2.5,3 and bladder cancer patients were significantly higher than that of Papanicolau level 1.Conclusion Positive rate of mutated p53 protein in serum was very high in bladder cancer patients,it could be served as an adjunct index for diagnosis of bladder cancer.更多还原