【作者】 纪卫东； 陈家堃； 吕嘉春； 吴中亮； 冯苏妹； 易菲；

【Author】 JI Wei-dong,CHEN Jia-kun,LU Jia-chun,WU Zhong-liangBIN Xiao-nong FENG Su-mei,YI Fei(Institute for Chemical Carcinogenesis,Guangzhou Medical College,Guangzhou 510182,China)

【机构】 广州医学院化学致癌研究所；

【摘要】 目的利用蛋白质组学技术识别结晶型NiS诱发人支气管上皮细胞恶性转化相关蛋白的差异表达,探讨镍致癌的分子机制。方法应用二维凝胶电泳与相应软件分析结晶型NiS诱发细胞恶性转化后蛋白质组变化,基质辅助激光解吸电离—飞行时间—质谱(MALDI-TOF-MS)结合数据库检索鉴定恶性转化相关蛋白。结果获得分辨率和重复性较好的二维凝胶电泳图谱,在分子量14.4～94 KD,等电点3～10范围内分离出约700个不同蛋白质点,对差异表达明显的等电点约6,分子量约25KD的蛋白质点进行质谱分析,鉴定出分子量为5.66,等电点为21.89KD的蛋白——硫氧还原蛋白过氧化物酶2 (Peroxiredoxin 2,PDX2),并发现该蛋白在恶性转化细胞中高表达。结论蛋白PDX2可能是参与了结晶型NiS诱发人支气管上皮细胞恶性转化的过程的重要蛋白分子,与细胞恶性转化有关。更多还原

【Abstract】 Objective To provide evidence for illustrating the molecular mechanism of nickel carcinogenesis, and to identify the differential expression of protein in crystalline NiS- induced neoplastic transformed cell by proteomics technology.Methods Two dimensional electrophoresis(2-DE) and the ImageMaster 3.01 software were used to analyze the differential protein expression in neoplastic transformed cell,matrix-assisted laser desorption/inoization-time of flight mass spectrometry(MALDI-TOF-MS) combined with database search was applied to identify protein PDX2 related to neoplastic transformation.Results the good 2DE pattern including resolution and reproducibility was obtained.Nearly 700 expressed proteins per 2-D gel were isolated with molecular weights(MW) ranging from14.4KD to 94 KD and pI 3-10.A protein PDX2 with MW 21.89KD, pI 5.66,which expressed a abundance in neoplastic transformed cell,was identified using MALDI-TOF-MS. Conclusion PDX2 was involved in neoplastic transformation of human bronchial epithelial cell induced by crystalline nickel sulfide.更多还原