【作者】 侯鑫； 刘永坚； 田丽霞；

【Author】 Xin Hou Yong-Jian Liu~* Li-Xia Tian Laboratory of Fish Nutrition,School of Life Sciences,SunYat-sen University,Guangzhou,China

【机构】 中山大学生命科学学院水生经济动物研究所；

【摘要】 本试验旨在研究实用饲料中石斑鱼对肌醇的最适需要量。本次试验饲料按肌醇添加量不同分为6组(0,50,150,300,500,1000 mg/kg),外加1组添加3%大豆卵磷脂组,一共7组饲料,每组3个重复(初始重为108.70±0.44g,n=3),实际测得肌醇含量为105,162,247, 440,672,1101,455 mg/kg。试验周期为8周,一天投喂2次,饱食投喂。肌醇含量247mg/kg组,其增重率显著高于对照组和162mg/kg组。肌醇含量440mg/kg组,其饲料效率和蛋白质效率比显著高于其他各组。肌醇含量≥440 mg/kg的4组,其肌醇在肝脏中的蓄积量显著高于前3组。各组饲料的增重率和肝脏中肌醇蓄积量通过折线模型进行分析,当饲料中肌醇含量约为222mg/kg时,增重率达到最大,当肌醇含量约为483mg/kg时,肝脏肌醇蓄积量达到最大。通过全鱼、肌肉、肝脏脂肪含量分析,表明饲料肌醇含量1101mg/kg组能显著降低脂肪的蓄积。对照组的蛋白质沉积率显著低于其他各组,672mg/kg组最高。1101mg/kg组脂肪沉积率显著低于其他各组。血清中甘油三酯含量各组间没有显著差异。试验结果表明,石斑鱼实用饲料中添加3%大豆卵磷脂可以满足鱼体正常生长的需要。更多还原

【Abstract】 An 8-week growth experiment was conducted to estimate the practical dietary requirement of inositol for grouper(Epinephelus coioides).Six diets were formulated to contain graded levels of supplemental inositol:0,50,150,300,500,1000 mg/kg diet and a diet contained 3%soya bean lecithin,providing 105,162,247,440,672,1101 and 455 mg/kg inosiol diet.Each diet was fed to triplicate groups of grouper(mean initial weight 108.70±0.44g,n=3).Fish fed 247 mg/kg inositol diet had significantly(p<0.05) higher weight gain ratio(WGR) than basal diet and 162 mg/kg inositol diet.The feeding efficiency(FE) and protein efficiency ratio(PER) were highest in fish fed 440 mg/kg inositol diet.The inositol concentrations in liver were highest in fish fed the>440 mg/kg inositol diets.WGR and inositol concentrations in the liver for the different treatments were analyzed by broken-line regression and indicated that the requirement for dietary inositol in growing grouper is about 229 mg/kg diet and 414 mg/kg.The lipid content of whole body,muscle and liver of grouper fed experimental diets with different inositol indicated the 1101 mg/kg inositol diet had significantly reduced lipid deposition.Fish fed 672mg/kg inositol diet had the significant highest net protein retention(NPR),and lowest in fish fed the basal diet.The net lipid retention(NLR) were lowest in fish fed the 1101 mg/kg inositol diet.Different diets had no significant effect on blood plasma triglyceride(TG). The results of this study indicated that the diet contained 3%soya bean lecithin was sufficient for the grouper normal growth..更多还原