【作者】 彭毛； 陈娟； 宋功武；

【Author】 PENG mao CHEN juan SONG gong-wu~* (Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules,Hubei University,Wuhan 430062)

【机构】 湖北大学有机功能分子合成与应用教育部重点实验室；

【摘要】 采用荧光,紫外光谱法研究了加替沙星(Gatifloxacin)、Fe(Ⅲ)与 DNA 三元体系的相互作用。结果表明,Fe(Ⅲ)和 DNA 均能猝灭 GTF 分子的荧光。测定了 GTF-Fe(Ⅲ)和 GTF-DNA 形成二元络合物反应的结合常数 K,结合位点数 n;绘制了 DNA 的工作曲线;考察了温度、溶液酸度对体系荧光强度的影响及离子干扰情况,发现 DNA 以静态猝灭的方式猝灭 GTF-Fe(Ⅲ)体系荧光。通过离子强度和热变性 DNA 实验的进一步研究揭示了加替沙星、Fe(Ⅲ)和 DNA 之间存在沟槽结合和静电作用。更多还原

【Abstract】 The interaction of Gatifloxacin(GTF),Fe(Ⅲ) and DNA were studied by fluorescent spectrum and UV-Vis spectrum.The result of the experiment suggested that the fluorescence of GTF can be quenched by both Fe(Ⅲ) and DNA.We studied the GTF-DNA and the GTF-Fe(Ⅲ) complexes by fluorescence quenched spectra,and the binding constant K and n were measured.Calibration curve of DNA was also determined.The effect of temperature,acidity and ion intensity on fluorescence intensity was studied.All of the experiments contributed to the further support of the mechanism of the combination of GTF,Fe(Ⅲ) with DNA,and it existed groove binding and static effect.更多还原