【作者】 陈娟； 李成芳； 宋功武；

【Author】 Chen Juan Li Chengfang Song Gongwu Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules,Hubei University

【机构】 湖北大学有机功能分子合成与应用教育部重点实验室；

【摘要】 基于2,2’-联吡啶芳环羟基化法,采用荧光光谱研究了胆红素与铜离子相互作用。研究了胆红素与铜离子反应产生羟基自由基（?）的具体过程,探讨了2,2’-联吡啶与胆红素及金属离子发生芳环羟基化反应的机理,发现胆红素与铜离子反应确实能产生羟基自由基,导致几乎没有荧光的2,2’-联吡啶发生芳环羟基化生成强荧光物质;加入自由基猝灭剂后,荧光强度的增强明显减弱。同时考察了各种参数如反应时间、酸度、Cu²⁺浓度、胆红素浓度,以及2’2’-联吡啶浓度等的影响。更多还原

【Abstract】 The interaction between bilirubin and copper ions was studied by fluorescence method using 2,2′-bipyridyl as a hydroxyl radical trapper through the hydroxylation of the aromatic compound.The mechanism of bilirubin binding with copper ions and the hydroxylation reaction was studied in details.It was found that bilirubin was able to bind to copper ions and produced hydroxyl radical surely.2,2′-bipyridyl,an almost non-fluorescence compound was transformed into a highly fluorescence substance on trapping hydroxyl radical,leading to a strong fluorescence increase.When the scavenging agent of hydroxyl radical was added to the system,the enhancement of fluorescence intensity of system decreased dramatically.The effects of reaction time,pH,and concentrations of bilirubin,copper ions and 2,2′-bipyridyl on the fluorescence intensity were studied.更多还原