【作者】 易平； 汪莉； 孙清萍； 朱英国；

【Author】 YI Ping WANGLi SUN Qing=Ping ZHU Ying-Guo~(**) (The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072. China)

【机构】 武汉大学生命科学学院植物发育生物学教育部重点实验室；

【摘要】 以红莲（HL）型水稻细胞质雄性不育系 A、保持系 B 及杂种一代 F₁为材料,首次比较研究了红莲型水稻线粒体 atp6基因转录本的编辑位点及各位点的编辑频率.结果表明 atp6基因的转录本有18个编辑位点,其中有15个发生在密码子的第一和第二位点上,这些位点的编辑最终会导致氨基酸种类的变化.18个编辑位点在 A、B 和 F₁中没有差异,但各位点的编辑频率在引入了核恢复基因的条件下发生了较大的变化,完全编辑的比例增加.这些结果首次证明 HL 型细胞质雄性不育与线粒体 atp6转录本的编辑有一定相关性,编辑不充分的转录产物最终会干扰线粒体功能的正常发挥.更多还原

【Abstract】 RNA editing is a process in which the genetic information of a gene transcript is changed during or after transcription. RNA editing exists extensively in the higher plant mitochondria, and is a necessary step for forming functional proteins. There may be some relationship between RNA editing and cytoplasmic male sterility （CMS）, a kind of phenomenon that is closely associated with mitochondrial genome mutations. The research materials were the gametopthyte male sterility line （A）,maintainer line （B） and F₁ hybrid （F₁） of HL-type CMS. cDNAs and DNAs of atp6 were obtained from A, B and F₁ by PCR and RT-PCR. Then sequences of cDNAs and DNAs are compared: A, B and F₁ share the same 15 editing sites found in the transcripts of atp6. The restorer gene in F₁ greatly changed the editing frequency of each editing site. So it is suggested that HL- type CMS is associated with RNA editing of atp6.更多还原