【作者】 顾玉成； 吴金平； 万进； 宋志红； 侯明生；

【Author】 Gu Yucheng~1 Wu Jinping~2 Wan Jin~1 Song Zhihong~1 Hou Mingsheng~2 (1. Biotechnology Institute of Hubei Academy of Agricultural Sciences, Wuhan 430064, China; 2. Huazhong Agricultural University College of Plant Science and Technology, Wuhan 430070, China)

【机构】 湖北省农科院生物技术研究所； 华中农业大学植科院；

【摘要】 本研究以魔芋顶芽、顶芽鳞片、侧芽、皮上芽苞、拟块茎鳞片为外植体,不同外植体在各自最适培养基上诱导。结果表明:拟块茎鳞片形成愈伤组织平均诱导率为90.6%,而侧芽形成愈伤细织平均诱导率只有43.3%。形成的愈伤组织在MS+6—BA(2.0mg/l)+NAA(0.5mg/l)+蔗糖(30g/l)培养基上进行不定芽诱导和扩繁,魔芋拟块茎鳞片不定芽的诱导率为96.4%,繁殖系数为4.9;而其他外植体最高的不定芽的诱导率为74.2%,繁殖系数为4.1。将形成的幼苗生根,在精心管理下,试管苗移栽的成活率可达100%。更多还原

【Abstract】 In this study, top buds, explants of Amorphophallus including bud scales, lateral buds, budlets and scales of artificial corm were induced to form calli on differemt media. The results demonstrated that the average ind ration rate of scales of artificial corm was 90.6%, whereas only 43.3% for the lateral buds. MS+BA (2.0mg/L)+NAA (0.01mg/L) was found to be suitable for the reproduction and induction of advantitions bud. The induction rate of advantitions buds on the scales of artificial corm was 96.4%, and the reproduction in crenses is 4.9. However, the highest induction rate of other explants is 74.2%, and the reproduction in crenses is just 4.1. Anyhow, after careful management on the culture tissue seedlings, their living rate can be as much as 100%.更多还原