【作者】 陈坤荣； 许泽永； 晏立英； 罗莉霞； 方小平； 张宗义； R G Birch； R G Dietzgen； C M Higgins；

【Author】 Chen Kunrong~1 Xu Zeyong~1 Yan Liying~1 Luo Lixia~1 Fang Xiaoping~1 Zhang Zongyi~1 R G Birch~2 R G Dietzgen~3 C M Higgins~3 (1. Oil Crops Research Institute, CAAS Wuhan 430062, China 2. The University of Queensland Brisbane 4072, Australia; 3. Queensland Agricultural Biotechnology Centre Brisbane 4072, Australia)

【机构】 中国农业科学院油料作物研究所农业部油料作物遗传改良重点实验室； 澳大利亚昆士兰大学； 澳大利亚昆士兰农业生物技术中心；

【摘要】 用中花3号花生胚性愈伤为受体,通过基因枪介导将花生条纹病毒壳蛋白基因(PStV CP)和潮霉素基因(Hyg~r)同时导入花生体胚。轰击胚经潮霉素筛选2～3月得到转化胚,其基因枪转化率为0.4%～2.9%。转化胚再生成花生植株。PCR检测PStV CP和Hyg~r均导入花生基因组中,两基因共转化率为50%。转基因花生农艺性状无明显改变,对PStV抗性表现不显著。这是国内通过基因枪介导获得转基因花生的首次报道。更多还原

【Abstract】 Coat protein (CP) gene of peanut stripe virus (PStV) and Hyg~r gene were cotransferred into embryogenic callus produced from mature seed of peanut variety Zhonghua No. 3 by particle bombardment. The embryos produced from died embryogenic callus after 2～3 months selection of bombarded callus on MS medium containing 20mg/L hygromycin B were regenerated into peanut plants. Frequency of transformation was 0.4%～2.9%. PCR test of To generation plants indicated the PStV CP gene and Hyg~r have been inserted into peanut genome. Transgenic peanut plants were normal in morphology and fertile. The resistance level of transgenic plants to PStV has no significant increment compared with non-transgenic plants by virus inoculation.更多还原