【作者】 李猛； 王永清； 田时炳； 罗章勇； 王小佳；

【Author】 Li Meng~1 Wang Yongqing~2 Tian Shibing~2 Luo Zhangyong~2 Wang XiaoJia~1 (1 College of Horticulture and Landscape,Southwest University,Chongqing 400716; 2 Chongqing Institute of Agricultural Sciences,Chongqing 400055)

【机构】 西南大学园艺园林学院； 重庆市农业科学研究所；

【摘要】 以茄子高感青枯病品种“三月茄”与高抗品种“S69”的 F1、F2为材料,对青枯病抗性遗传进行了分析,同时开展了与抗性基因连锁的 AFLP 标记鉴定。结果表明：“S69”对青枯病的抗病性属于不完全隐性遗传,感病性属于不完全显性；抗性由1-2对基因控制。对86个 F2单株 AFLP 分析,鉴定出1个与“S69”抗性基因连锁的 AFLP 标记39A980(该引物组合为 E-ACC／M-CTG),该标记与抗性基因间的交换值为4．65％,遗传距离为4．9cM。更多还原

【Abstract】 F1 and F2 populations,derived from the cross between bacterial wilt highly susceptible cuhivar‘Sanyueqie’and highly resistant Indonesian cultivar‘S69’,were used for investigation of the inheritance of resistance to bacteria wilt and for identification of associated AFLP markers in egg- plant.The results showed that the resistance to bacterial wilt was incompletely recessive,otherwise susceptibility was incompletely dominant,which was controlled by 1-2 genes.By using a combina- tion of the amplified fragment length polymorphism (AFLP)technique and bulked segregant analysis (BSA),an AFLP marker,named 39A980 was identified with the primer combination E-ACC/M -CTG to be linked to the major resistant gene.The crossing-over value and the genetic distance between the resistant gene and‘39A980’were estimated as 4.65% and 4.9cM respectively by ana- lyzing a set of 86 F2 plants.更多还原