【作者】 罗璨； 吴芹； 黄燮南； 孙安盛； 石京山；

【Author】 LUO Can, WU Qin, HUANG Xie-Nan, SUN An-Sheng, SHI Jing-Shan Department of Pharmacology, Zunyi Medical College, Zunyi 563003, China

【机构】 遵义医学院药理学教研室；

【摘要】 目的观察银杏叶提取物(GbE)对正常大鼠海马内caspase-3和β淀粉样肽原蛋白(APP)水平的影响。方法不同年龄组大鼠灌胃给予GbE100mg／kg后14天,采用免疫组织化学染色、图象分析系统定量分析测定大脑海马区caspase-3和APP的表达情况。结果在3个不同的年龄组大鼠的海马区,给予GbE组caspase-3和APP 水平均明显高于对照组。结论 GbE能提高大鼠大脑海马区caspase-3和APP水平,其抗阿尔采未病的意义值得进一步研究。更多还原

【Abstract】 AIM: To explore the effect of Ginkgo biloba extract (GbE) on the levels of caspase-3 and amyloid precursor protein (APP) in normal rats’ hippocampus. METHODS: Iramunohistochemistry method was used for qualitative analysis of the expressions of caspase-3 and APP, and an image analysis method was used for the quantification of the levels of caspase-3 and APP after GbE was administered to rats of different ages for 14 d. RESULTS: The mean absorbance of staining of caspase-3 and APP was markedly higher in GbE group than that in control groups. The expressions of caspase-3 and APP were intensified in the hippocampus of rats after GbE administration. CONCLUSION: GbE can raise the levels of caspase-3 and APP in the hippocampus of normal rats.更多还原