【作者】 杨汝德； 陈琼； 陈惠音；

【Author】 Yang Rude Chen Qiong Chen Huiying (College of Food & Bioengineering,South China University ofTech.,Guangzhou 510641)

【机构】 华南理工大学食品与生物工程学院； 广东化工制药职业技术学院；

【摘要】 从15天龄健康小鸡盲肠中分离筛选出鸡双歧杆菌 B171,其最佳培养条件为初始 pH 值7.0,最适培养温度40℃。采用正交实验确定的优化培养基配方,双歧杆菌 B171的增殖菌数为6.2×10~8。双歧杆菌 B171在体外对三种鸡肠道致病菌均有明显的抑制作用。双歧杆菌 B171对丁胺卡那霉素、新霉素、巴龙霉素耐药,对青霉素、万古霉素、林可霉素和杆菌肽敏感。更多还原

【Abstract】 Using BS medium,the Bifidobacterium pullorum was isolated from the appendix of fifteen-day-old chicken.The optimized fermentation conditions were:pH value of media 7.0,temperature 40℃.Using orthogonality test,the optimal prescription for fermentation were composed of 0.9% yeast extract,0.6% tryptone,0.6% soya peptone,2.5% glucose,0.2% bifidus,10% growth factor.The terminal time was at 18 hours.The highest biomass concentration obtained experiments was 6.2×10~8/mL.The growth of Entropathogen had been inhibited by B171. B171 had drug-resistance to amikacin,neomycin,paromomycin,and was sensitive to penicillin,vancomycin,lincomycin and bacitracin.更多还原