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短双歧杆菌增殖培养基的优化研究

Optimization Study of Stimulated Culture of Bifidobacterium Breve

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【作者】 陈惠音肖仔君杨汝德

【Author】 Chen Huiyin Xiao Zijun Yang Rude (College of Food & Bioengineering,South China Univ.ofTech.,Guangzhou510640)

【机构】 华南理工大学食品与生物工程学院韶关学院食品系

【摘要】 本研究针对短双歧杆菌的营养需要,采用正交实验设计优化增殖培养基,结果表明,酵母膏是短双歧杆菌的显著影响因素, 最佳的优化培养基配比是:酵母膏0.9%,胰蛋白胨0.6%,大豆蛋白胨0.6%,葡萄糖0.6%,双歧因子0.2%,生长因子10%。用经优化的增殖培养基配方测定短双歧杆菌的生长曲线及其 pH 的变化,确定该菌增殖培养的最适终止时间约为18h,此时平板菌落计数的菌数可高达1.5×1010cfu/ml。

【Abstract】 According to the nutritional needs of B.breve,based culture and stimulated factors were optimized by quadratic design.The optimum based culture was as following:yeast extract was 0.9%,tryptone was 0.6%,soya peptone was 0.6%,glucose was 0.6%,bifidus was 0.2%,and growth factor was 10%.According the determining result of growth curve of B.breve and the pH change of the optimized stimulated culture,the terminal time was at 18 hours.B.breve got to 1.5×1010cfu/ml in the culture for 18 hours at 37℃ under anaerobic condition.

【关键词】 短双歧杆菌增殖培养基优化
【Key words】 B.breveOptimization of culture
  • 【会议录名称】 第三届“益生菌、益生元与健康研讨会”论文集
  • 【会议名称】第三届“益生菌、益生元与健康研讨会”
  • 【会议时间】2004
  • 【分类号】TS201.3
  • 【主办单位】广东省食品学会
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