【作者】 钱春燕； 郭启华； 谷学新； 邹洪；

【Author】 QIAN Chun-yan,GUO Qi-hua,GU Xue-xin,ZOU Hong(Department of Chemistry .Capital Normal University ,Beijin 1000S7,China)

【机构】 首都师范大学化学系；

【摘要】 以槲皮素为定量代表,建立了槲皮素-钼（Ⅵ）-溴化十六烷基三甲铵（CTMAB）三元络合物体系测定银杏样品中的总黄酮。在pH4.0的HAc-NaAc缓冲体系中,槲皮素-钼（Ⅵ）-CTMAB可形成浅黄色二元络合物,该络合物在428 nm处有最大吸收峰,摩尔吸光系数为1.95×10⁴L·mol^-1·cm^-1,槲皮系的浓度在0～4×10^-5mol·L^-1范围内线性关系良好,检出限为6.15×10^-8mol·L^-1,方法用于银杏茶和银杏成药中总黄酮的测定,加标回收率为98.5%～116.2%,方法简便,结果满意。更多还原

【Abstract】 A color reaction of quercetin (Qu) with molybdenum( Ⅵ ) in the presence of cetyl trimethylam-monium bromide (CTMAB) was proposed. In HAc-NaAc buffer medium at pH4. 0,the yellow complex of Qu-Mo ( Ⅵ )-CTMAB has a maximum absorption at 428 nm and its apparent molar absorptivity is 1. 95×104 L · mol-1 · cm-1. Beer’s law is obeyed in the concentration range of 0-4X10-5 mol · L-1 for the quercetin and the limit of detection is 6. 15 × 10-8 mol · L-1. This method has been applied to the spectrometric determination of flavonoids in the ginkgo materials with the recovery of 98. 5% -116. 2%.更多还原