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一步二温式RT-PCR检测南美白对虾桃拉综合症病毒
Detection of taura syndrome virus Litopetiaeus vannamei by one-step and two-temperature reverse transcription polymerase chain reaction (RT-PCR)
【作者】 陈晓汉; 曾地刚; 彭敏; 雷爱莹; 李咏梅; 蒋伟明;
【Author】 CHEN Xiao-han,ZENG Di-gang,PENG Min,LEI Ai-ying,LI Yong-mei,JIANG Wei-ming (Fisheries Institute of Guangxi, Guangxi Nanning 530021, China)
【机构】 广西水产研究所;
【摘要】 建立一种一步法和二温式的逆转录聚合酶链式反应,用于扩增南美白对虾桃拉综合症病毒231bp的特异基因片断,对300份南美白对虾临床样品的检测结果表明,这是一种快速和敏感的桃拉综合症病毒诊断方法。
【Abstract】 A one-step and two-temperature reverse transcription polymerase chain reaction (RT-PCR) .which could amplify 231 bp of fhe genome of taura symdrome virus (TSV)in Litope-naeus vannamei shrimp was developed and optimized.The test of 300 clinical L.vannamei shrimp samples showed that one-step two-temperature RT-RCR.could be used as a rapid and sensitive detection method for TSV diagnosis
【Key words】 Litopenenaeus vannamei; taura syndrome virus; one-step and two-temperature RT-PCR;
- 【会议录名称】 广西水产研究所论文集(2001—2005)
- 【会议时间】2006-10
- 【分类号】S945