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茉莉酸诱导玉米地下部对化学防御反应影响的时间和浓度效应

Timing and concentration effects of external application of jasmonic acid to the belowground on the chemical defense response in maize

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【作者】 冯远娇王建武骆世明

【Author】 FENG Yuan-jiao WANG Jian-wu LUO Shi-ming Institute of Tropical and Subtropical Ecology,South China Agricultural University,Guangzhou 510642,China; Key Laboratory of Ecological Agriculture of Ministry of Agriculture,South China Agricultural University,Guangzhou 510642,China; Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions,South China Agricultural University,Guangzhou 510642,China

【机构】 华南农业大学热带亚热带生态研究所华南农业大学农业部生态农业重点开放实验室广东省高等学校农业生态与农村环境重点实验室

【摘要】 本文通过在"高油115"玉米地下部施用4种不同浓度(10μM、50μM、100μM、200μM)的茉莉酸3~48 h后,采用生化成分分析和基因表达分析方法,检测了处理部位(根系)和非处理部位(叶片)中防御物质及防御相关基因的表达,从而探讨茉莉酸诱导玉米地下部对化学防御反应影响的时间和浓度效应。结果表明,茉莉酸处理玉米地下部对处理部位(根系)和非处理部位(叶片)化学防御反应的影响均与茉莉酸作用的时间和浓度相关。茉莉酸处理玉米地下部后,在3~12 h内就能直接诱导处理部位(根系)Bx9、PAL、PR-2a、MPI和FPS基因的表达,使得根中的丁布含量增加而总酚含量下降,同时100μM茉莉酸处理浓度产生的诱导作用最强,其次是50μM,再次是10μM;到了24~48 h时,诱导作用逐渐减弱,降低作用有所增加,仅使根中防御基因PR-1、PR-2a和MPI表达量增加,而PAL基因的表达量、丁布含量和总酚含量则有所下降,并有随处理浓度的升高而增强的趋势。另外,茉莉酸处理玉米地下部还能间接影响到非处理部位(叶片)的化学防御反应,50μM茉莉酸在处理后3 h就能系统诱导叶片中Bx9和FPS基因的表达,使叶片的丁布含量增加;在6~24 h内则使叶片Bx9、PAL、PR-1、MPI和TPS基因表达量增加,丁布和总酚含量降低,并随着时间的推移,起作用的茉莉酸浓度逐渐升高;到了48 h,个别浓度的茉莉酸处理还能使叶片中PR-2a和PAL基因的表达量增加。可见,对于大部分检测指标来说,茉莉酸处理玉米地下部对地上部的间接诱导作用不如对地下部的直接诱导作用强,根系启动防御反应的时间较叶片早,同时,随着处理浓度的增加,茉莉酸对根系和叶片中防御反应的诱导作用有增强的趋势。

【Abstract】 In order to explore the timing and concentration effects of exogenous jasmonic acid application to the belowground on the chemical defense in both aboveground and belowground parts of maize,biochemical analysis and gene expression analysis methods were employed to detect the defense chemicals content and defense-related genes expression in both leaves and roots of maize cultivar Gaoyou 115 after its roots were treated with 10μM,50μM,100μM and 200μM jasmonic acid for 3~48 h.The results showed that the chemical defense response in both treated part(roots) and non-treated part(leaves) was associated with the time span and concentration of jasmonic acid application.Immediately after jasmonic acid treatment to the roots for 3~12 h,the gene expression of Bx9,PAL,PR-2a,MP land FPS in the treated part was directly induced, resulting in an increase of DIMBOA and decrease of total phenolics;meanwhile,the most strong induced effects were observed at the jasmonic acid concentration of 100μM,followed by 50μM, and later 10μM.After the roots were treated withjasmonic acid for 24~48 h,the inducible effects appeared to be weaker and only the gene expression of PR-1,PR-2a and MPI became stronger, whereas gene expression of PAL,DIMBOA content and total phenolics content were reduced, showing an increasing tendency with the increase of jasmonic acid concentrations.Besides,the treatment ofjasmonic acid to the roots could indirectly affect the chemical defense response in the non-treated part(leaves) of maize.After the roots were applied with 50μM for 3 h,gene expression of Bx9and FPS was induced systematically,which caused an consequent increase of the DIMBOA content in the leaves;for 6~24 h,gene expression of Bx9,PAL,PR-1,MPI and TPS in the leaves was enhanced,while DIMBOA content and total phenolics content were declined. The contributing concentrations of jasmonic acid tended to increase with the prolongation of experimental period,and some certain concentrations would still lift up the gene expression of PR-2a and PAL in the leaves after 48 h.It was obvious that for most parameters measured in the present study,the directly-induced effects of jasmonic acid application to the roots on the belowground are much more significant than the indirect induction on the aboveground,and that roots will start the defense response earlier that the leaves;moreover,the inducible effects of jasmonic acid on the roots and leaves can be increased with the increasing of jasmonic concentrations.

  • 【会议录名称】 第五届中国青年生态学工作者学术研讨会论文集
  • 【会议名称】第五届中国青年生态学工作者学术研讨会
  • 【会议时间】2008-11
  • 【会议地点】中国广东广州
  • 【分类号】S513
  • 【主办单位】中国生态学会青年工作委员会
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