【作者】 肖武名； 杨祁云； 陈志强； 王慧； 郭涛； 刘永柱； 朱小源；

【Author】 XIAO Wu-ming~(1.2) YANG Qi-yun~(1*) CHEN Zhi-qiang~(2*) WANG Hui~2 GUO Tao~2 LIU Yong-zhu~2 ZHU Xiao-yuan~1 1. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640; 2. College of Agriculture, South China Agricultural University, Guangzhou 510642

【机构】 广东省农业科学院植物保护研究所； 华南农业大学农学院；

【摘要】 本研究采用38个稻瘟病菌代表性菌株对中感稻瘟病的水稻品种"中二软占"空间诱变 SP₅代（诱变5代）品系 Z1、Z3及 Z6等进行抗谱测定,发现不同诱变品系的抗谱不同,可从空间诱变后代筛选出具有广谱抗性的突变品系。同时,采用 SSR 引物对抗性水平依次为广谱、中等抗谱、抗谱窄的3个诱变品系与原品种对照进行多态性分析,比较诱变品系的基因组突变情况,发现抗谱较广的诱变品系,其突变频率相对要高,并且在不同染色体的突变频率并不相同。更多还原

【Abstract】 The resistance spectrum to blast isolates of six spaced-induced lines of SP₅ generation, Z1, Z3 and Z6 etc, derived from rice cultivar Zhong-er-ruan-zhan which was moderately susceptible to rice blast, were tested with 38 representative blast isolates. It was found that different mutant lines showed different resistance spectrum to blast isolates, and mutant lines with broad-spectrum could be selected from the progeny of rice after space mutation. Meanwhile, the genomes of three mutant lines with broad, mediate and narrow resistance spectrum respectively and the original cultivar were scanned with SSR primers covering whole rice genome, the genomic mutagenesis of mutant lines was analyzed as compared with the original cultivar, the findings displayed that the mutant frequencies were larger in mutant lines showing broader resistance spectrum. The results also demonstrated the mutant frequencies on different chromosomes varied depending on mutant lines.更多还原