【作者】 马金骏； 冒维维； 朱玉英； 陈学好； 薄天岳；

【机构】 上海市农业科学院园艺研究所上海市农业科学院设施园艺重点实验室； 扬州大学园艺与植物保护学院；

【摘要】 利用RAPD、ISSR和SRAP三种分子标记技术,对两个不结球白菜杂交组合及其各自亲本进行分析。结果表明3个RAPD引物、3个ISSR引物和3对SRAP引物可扩增出组合1双亲的互补特征带,将双亲和F₁区分开来;3个ISSR引物和2对SRAP引物可扩增出组合2双亲的互补特征带,将双亲和F₁区分开来。从而建立了由RAPD、ISSR和SRAP三种标记技术组成的鉴定两份不结球白菜杂交组合的分子指纹图谱,并转化为数字指纹图谱,为不结球白菜种子纯度的鉴定开辟了新的技术途径,为新品种知识产权的保护提供了重要手段。更多还原

【Abstract】 Two hybrids and their parents of non-heading Chinese cabbage were analyzed by using three molecular markers,RAPD,ISSR and SRAP.The results indicated that three RAPD primers and three ISSR primers and three pairs of SRAP primers could generate parental complementary characteristic bands for the group one;three ISSR primers and two pairs of SRAP primers could generate parental complementary characteristic bands for the group two. The molecular fingerprint of two hybrids of non-heading Chinese cabbage was established,and then transformed into digital fingerprints.It provided a new technology and an important means for identification of seed purity and protection of intellectual property of new varieties.更多还原