【作者】 王燕； 翟欣； 朱长志； 龚义勤； 曾琦； 柳李旺；

【机构】 南京农业大学作物遗传与种质创新国家重点实验室园艺学院； 温州市农科院蔬菜科学研究所；

【摘要】 利用相关序列扩增多态性(SRAP)和随机扩增多态性DNA(RAPD)标记技术对25个茄子品种进行了指纹图谱与遗传多样性分析。利用18个SRAP引物组合共产生107条谱带。其中多态性谱带51条,多态性比率为47.7%;筛选出5个多态性RAPD引物,获得28条多态性条带。分别利用两种分子标记技术对25个茄子品种进行了品种分子标记指纹图谱的绘制。RAPD双引物rp351-rp403,RAPD三引物组合rp335-rp354- rp403,SRAP三引物组合ga18-em1+fc8-em1+me6-1-em14-1可以将25个品种完全区分开。基于SRAP和RAPD标记的聚类分析结果表明,25个茄子品种按果色可分为4大类,一定程度上能够揭示品种之间园艺学性状的相似性及亲缘关系远近。更多还原

【Abstract】 Fingerprinting and genetic diversity analysis of 25 eggplant cultivars in China were conducted with SRAP and RAPD markers.18 SRAP primer combinations were used to amplify and 107 bands were generated with 51 of them being polymorphic.A total of 28 polymorphic bands were generated by five RAPD primers.The fingerprinting of 25 cultivars was constructed by SRAP and RAPD markers.The primer capacity of distinguishing the 25 cultivars was tested.Two RAPD primers of rp351-rp403,three RAPD primers of rp335-rp354-rp403,and SRAP three primer combinations of ga18-em1+fc8-eml+me6-1-em14-1 could discriminate all the 25 eggplant cultivars effectively.According to the result of cluster from the SRAP and RAPD analysis,25 eggplant cultivars can be grouped into four clusters,which is in accordance with the horticultural taxomy and relaionships among all cultivars.更多还原